Veth M, Sonnenbichler J
Anal Biochem. 1986 Feb 15;153(1):54-6. doi: 10.1016/0003-2697(86)90060-6.
For a quick analytical identification of histones a two-dimensional electrophoretic system has been developed. First the proteins are separated on cellulose acetate strips in alkaline buffer. Then they are reelectrophoresed in a second dimension on polyacrylamide gels either with sodium dodecyl sulfate or in the presence of urea at pH 2.7. The technique is demonstrated with yeast and calf thymus histones.
为了快速分析鉴定组蛋白，已开发出一种二维电泳系统。首先，蛋白质在碱性缓冲液中的醋酸纤维素条上进行分离。然后，它们在第二维中在聚丙烯酰胺凝胶上进行再电泳，要么使用十二烷基硫酸钠，要么在pH 2.7的尿素存在下进行。该技术已用酵母和小牛胸腺组蛋白进行了演示。
