酵母组蛋白在纤维素薄层层析条和聚丙烯酰胺凝胶上的双向电泳。
Two-dimensional electrophoresis of yeast histones on cellogel strips and polyacrylamide gels.
作者信息
Veth M, Sonnenbichler J
出版信息
Anal Biochem. 1986 Feb 15;153(1):54-6. doi: 10.1016/0003-2697(86)90060-6.
PMID:3516008
Abstract
For a quick analytical identification of histones a two-dimensional electrophoretic system has been developed. First the proteins are separated on cellulose acetate strips in alkaline buffer. Then they are reelectrophoresed in a second dimension on polyacrylamide gels either with sodium dodecyl sulfate or in the presence of urea at pH 2.7. The technique is demonstrated with yeast and calf thymus histones.
摘要
为了快速分析鉴定组蛋白，已开发出一种二维电泳系统。首先，蛋白质在碱性缓冲液中的醋酸纤维素条上进行分离。然后，它们在第二维中在聚丙烯酰胺凝胶上进行再电泳，要么使用十二烷基硫酸钠，要么在pH 2.7的尿素存在下进行。该技术已用酵母和小牛胸腺组蛋白进行了演示。
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