在不存在去污剂的情况下，通过组蛋白在酸性尿素凝胶中的电泳迁移率来进行组蛋白的测定。
Mr determination of histones from their electrophoretic mobility in acidic urea gels in the absence of detergents.
作者信息
Brandt W F, Sewell B T, von Holt C
出版信息
FEBS Lett. 1986 Jan 6;194(2):273-7. doi: 10.1016/0014-5793(86)80099-0.
PMID:3940898
Abstract
The Mr of histones can be determined from their electrophoretic mobility at pH 2.3, 8 M urea in a polyacrylamide gel by correcting for differences in their charge density and properties of the gel matrix. The applicability of this method to other proteins is considered.
摘要
组蛋白的相对分子质量（Mr）可通过在pH 2.3、含8M尿素的聚丙烯酰胺凝胶中进行电泳迁移率测定，并校正其电荷密度和凝胶基质性质的差异来确定。本文还考虑了该方法对其他蛋白质的适用性。
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