Kawai Mariko Yamamoto, Ozasa Ryosuke, Ishimoto Takuya, Nakano Takayoshi, Yamamoto Hiromitsu, Kashiwagi Marina, Yamanaka Shigeki, Nakao Kazumasa, Maruyama Hiroki, Bessho Kazuhisa, Ohura Kiyoshi
Department of Welfare, Kansai Women's College, Osaka 582-0026, Japan.
Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.
Materials (Basel). 2022 Jan 27;15(3):993. doi: 10.3390/ma15030993.
The application of periodontal tissue in regenerative medicine has gained increasing interest since it has a high potential to induce hard-tissue regeneration, and is easy to handle and graft to other areas of the oral cavity or tissues. Additionally, bone morphogenetic protein-2 (BMP-2) has a high potential to induce the differentiation of mesenchymal stem cells into osteogenic cells. We previously developed a system for a gene transfer to the periodontal tissues in animal models. In this study, we aimed to reveal the potential and efficiency of periodontal tissue as a biomaterial for hard-tissue regeneration following a gene transfer. A non-viral expression vector carrying was injected into the palate of the periodontal tissues of Wistar rats, followed by electroporation. The periodontal tissues were analyzed through bone morphometric analyses, including mineral apposition rate (MAR) determination and collagen micro-arrangement, which is a bone quality parameter, before and after a gene transfer. The MAR was significantly higher 3-6 d after the gene transfer than that before the gene transfer. Collagen orientation was normally maintained even after the gene transfer, suggesting that the gene transfer has no adverse effects on bone quality. Our results suggest that periodontal tissue electroporated with could be a novel biomaterial candidate for hard-tissue regeneration therapy.
由于牙周组织在诱导硬组织再生方面具有很高的潜力，并且易于操作和移植到口腔或组织的其他区域，因此其在再生医学中的应用越来越受到关注。此外，骨形态发生蛋白-2（BMP-2）具有很高的潜力可诱导间充质干细胞分化为成骨细胞。我们之前在动物模型中开发了一种向牙周组织进行基因转移的系统。在本研究中，我们旨在揭示牙周组织作为基因转移后硬组织再生生物材料的潜力和效率。将携带[具体基因]的非病毒表达载体注射到Wistar大鼠牙周组织的腭部，随后进行电穿孔。在基因转移前后，通过骨形态计量学分析对牙周组织进行分析，包括矿物质沉积率（MAR）测定和作为骨质量参数的胶原微排列。基因转移后3 - 6天的MAR显著高于基因转移前。即使在基因转移后，胶原取向仍正常维持，这表明基因转移对骨质量没有不良影响。我们的结果表明，用[具体基因]进行电穿孔的牙周组织可能是硬组织再生治疗的一种新型生物材料候选物。
