Instituto de Microbiología, Universidad San Francisco de Quito USFQ, Diego de Robles y Via Interoceánica, Quito 17-1200-841, Ecuador.
Colegio de Ciencias e Ingeniería, Instituto Biósfera, Universidad San Francisco de Quito USFQ, Diego de Robles y Vía Interoceánica, Quito 17-1200-841, Ecuador.
Int J Environ Res Public Health. 2022 Jan 28;19(3):1484. doi: 10.3390/ijerph19031484.
Biological treatment using sulfate-reducing bacteria (SRB) is a promising approach to remediate acid rock drainage (ARD). Our purpose was to assess the performance of a sequential system consisting of a limestone bed filter followed by a sulfate-reducing bioreactor treating synthetic ARD for 375 days and to evaluate changes in microbial composition. The treatment system was effective in increasing the pH of the ARD from 2.7 to 7.5 and removed total Cu(II) and Zn(II) concentrations by up to 99.8% and 99.9%, respectively. The presence of sulfate in ARD promoted sulfidogenesis and changed the diversity and structure of the microbial communities. spp. was the most abundant amplicon sequence variant (ASV); however, methane production was not detected. Biodiversity indexes decreased over time with the bioreactor operation, whereas SRB abundance remained stable. , , and became more abundant, while , and decreased. and were enriched with bioreactor operation time. At the beginning, ASVs with relative abundance <2% represented 65% of the microbial community and 21% at the end of the study period. Thus, the results show that the microbial community gradually lost diversity while the treatment system was highly efficient in remediating ARD.
利用硫酸盐还原菌（SRB）进行生物处理是修复酸性矿山排水（ARD）的一种很有前途的方法。我们的目的是评估由石灰石床过滤器和硫酸盐还原生物反应器组成的顺序系统在处理合成 ARD 方面的性能，持续 375 天，并评估微生物组成的变化。该处理系统有效地将 ARD 的 pH 值从 2.7 增加到 7.5，并分别去除了高达 99.8%和 99.9%的总 Cu(II)和 Zn(II)浓度。ARD 中存在的硫酸盐促进了硫化作用，并改变了微生物群落的多样性和结构。 spp. 是最丰富的扩增子序列变异（ASV）；然而，没有检测到甲烷的产生。随着生物反应器的运行，生物多样性指数随时间下降，而 SRB 的丰度保持稳定。 、 、 和 变得更加丰富，而 、 和 减少。 和 在生物反应器运行时间的富集。开始时，相对丰度 <2%的 ASVs 代表微生物群落的 65%，而在研究结束时占 21%。因此，结果表明，微生物群落的多样性逐渐丧失，而处理系统在修复 ARD 方面非常高效。
Zotero 插件
