College of Animal Science, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, China.
Int J Mol Sci. 2022 Jan 26;23(3):1413. doi: 10.3390/ijms23031413.
(1) Background: As a novel type of non-coding RNA with a stable closed-loop structure, circular RNA (circRNA) can interact with microRNA (miRNA) and influence the expression of miRNA target genes. However, circRNA involved in pituitary growth hormone (GH) regulation is poorly understood. Our previous study revealed protein kinase C alpha () as the target gene of miR-709. Currently, the expression and function of rno_circRNA_0001004 in the rat pituitary gland is not clarified; (2) Methods: In this study, both bioinformatics analysis and dual-luciferase report assays showed a target relationship between rno_circRNA_0001004 and miR-709. Furthermore, the rno_circRNA_0001004 overexpression vector and si-circ_0001004 were constructed and transfected into GH cells; (3) Results: We found that rno_circRNA_0001004 expression was positively correlated with the PRKCA gene and GH expression levels, while it was negatively correlated with miR-709. In addition, overexpression of rno-circ_0001004 also promoted proliferation and relieved the inhibition of miR-709 in GH cells; (4) Conclusions: Our findings show that rno_circ_0001004 acts as a novel sponge for miR-709 to regulate GH synthesis and cell proliferation, and are the first case of discovery of the regulatory role of circRNA_0001004 in pituitary GH.
(1) 背景：环状 RNA（circRNA）作为一种新型的非编码 RNA，具有稳定的闭环结构，可与 microRNA（miRNA）相互作用，影响 miRNA 靶基因的表达。然而，调节垂体生长激素（GH）的 circRNA 知之甚少。我们之前的研究表明蛋白激酶 C 阿尔法（PKCα）是 miR-709 的靶基因。目前，大鼠垂体中 rno_circRNA_0001004 的表达和功能尚不清楚；(2) 方法：在这项研究中，生物信息学分析和双荧光素酶报告实验均显示 rno_circRNA_0001004 与 miR-709 之间存在靶关系。此外，构建了 rno_circRNA_0001004 过表达载体和 si-circ_0001004 并转染 GH 细胞；(3) 结果：我们发现 rno_circRNA_0001004 的表达与 PRKCA 基因和 GH 表达水平呈正相关，而与 miR-709 呈负相关。此外，rno-circ_0001004 的过表达也促进了 GH 细胞的增殖，并缓解了 miR-709 的抑制作用；(4) 结论：我们的研究结果表明，rno_circ_0001004 作为 miR-709 的新型海绵分子，调节 GH 的合成和细胞增殖，这是首次发现 circRNA_0001004 在垂体 GH 中的调节作用。
