Department of Laboratory Animals, College of Animal Sciences, Jilin University, Changchun, Jilin, P.R. China.
PLoS One. 2019 Sep 11;14(9):e0222340. doi: 10.1371/journal.pone.0222340. eCollection 2019.
Growth hormone (GH) is an important hormone released by the pituitary gland that plays a key role in the growth and development of organisms. In our study, TargetScan analysis and the dual luciferase reporter assays were used to predict and screen for miRNAs that might act on the rat Gh1 gene, and we identified miR-543-5p. Then, the GH3 cell line and the primary rat pituitary cells were transfected with miRNA mimic, inhibitor, and siRNA. We detected the Gh1 gene expression and the GH secretion by real-time PCR and ELISAs, respectively, to verify the regulatory effect of miR-543-5p on GH secretion. The results showed that miR-543-5p can inhibit Gh1 mRNA expression and reduce GH secretion. MiR-543-5p inhibitor upregulated Gh1 mRNA expression and increased GH secretion compared with the negative control. In summary, miR-543-5p downregulates Gh1 expression, resulting in a decrease in GH synthesis and secretion, which demonstrates the important role of miRNAs in regulating GH and animal growth and development.
生长激素（GH）是由垂体分泌的一种重要激素，在生物体的生长和发育中起着关键作用。在我们的研究中，使用 TargetScan 分析和双荧光素酶报告基因检测实验来预测和筛选可能作用于大鼠 Gh1 基因的 miRNAs，我们鉴定到 miR-543-5p。然后，使用 miRNA 模拟物、抑制剂和 siRNA 转染 GH3 细胞系和原代大鼠垂体细胞。我们通过实时 PCR 和 ELISA 分别检测 Gh1 基因表达和 GH 分泌，以验证 miR-543-5p 对 GH 分泌的调节作用。结果表明，miR-543-5p 可以抑制 Gh1 mRNA 表达并减少 GH 分泌。与阴性对照相比，miR-543-5p 抑制剂上调 Gh1 mRNA 表达并增加 GH 分泌。总之，miR-543-5p 下调 Gh1 表达，导致 GH 合成和分泌减少，这表明 miRNAs 在调节 GH 和动物生长发育方面发挥着重要作用。
