Research Institute of Integrative Life Sciences, Dongguk University, Goyang-si 10326, Korea.
Department of Medical Biotechnology (BK21 Plus Team), Dongguk University, Goyang-si 10326, Korea.
Int J Mol Sci. 2022 Jan 28;23(3):1522. doi: 10.3390/ijms23031522.
Human umbilical-cord-derived mesenchymal stem cells (hUC-MSC) are a type of mesenchymal stem cells and are more primitive than other MSCs. In this study, we identify novel genes and signal-activating proteins involved in the neural differentiation of hUC-MSCs induced by Low-Intensity Sub-Sonic Vibration (LISSV). RNA sequencing was used to find genes involved in the differentiation process by LISSV. The changes in hUC-MSCs caused by LISSV were confirmed by overexpression and gene knockdown through small interfering RNA experiments. The six genes were increased among genes related to neurons and the nervous system. One of them, the gene, is known to play a role as a guide for axons in the development of the nervous system. When the PLXNA4 recombinant protein was added, neuron-related genes were increased. In the gene knockdown experiment, the expression of neuron-related genes was not changed by LISSV exposure. The gene is activated by sema family ligands. The expression of was increased by LISSV, and its downstream signaling molecule, FYN, was also activated. We suggest that the gene plays an important role in hUC-MSC neuronal differentiation through exposure to LISSV. The differentiation process depends on --dependent FYN activation in hUC-MSCs.
人脐带间充质干细胞（hUC-MSC）是一种间充质干细胞，比其他 MSC 更原始。在这项研究中，我们鉴定了参与低强度亚声振动（LISSV）诱导 hUC-MSC 神经分化的新基因和信号激活蛋白。通过 RNA 测序找到了与 LISSV 诱导的分化过程相关的基因。通过小干扰 RNA 实验中的过表达和基因敲低实验，证实了 LISSV 对 hUC-MSCs 的影响。在与神经元和神经系统相关的基因中，有六个基因增加。其中一个基因，即基因，已知在神经系统发育中作为轴突的导向物发挥作用。当添加 PLXNA4 重组蛋白时，与神经元相关的基因增加。在基因敲低实验中，LISSV 暴露并没有改变与神经元相关的基因的表达。基因是由 sema 家族配体激活的。LISSV 增加了的表达，其下游信号分子 FYN 也被激活。我们认为，在 hUC-MSC 神经元分化过程中，基因通过暴露于 LISSV 发挥重要作用。分化过程取决于 hUC-MSCs 中依赖的 FYN 激活。
