Dongguk University Research Institute of Biotechnology, Seoul 100-715, Republic of Korea.
Life Sci. 2012 Apr 20;90(15-16):591-9. doi: 10.1016/j.lfs.2012.02.014. Epub 2012 Mar 3.
Adult stem cells, such as umbilical cord-derived mesenchymal stem cells (UC-MSCs), have the potential to differentiate into various types of cells, including neurons. Research has shown that mechanical stimulation induces a response in MSCs, specifically, low and high intensity sub-sonic vibration (SSV) has been shown to facilitate wound healing. In this study, the effects of SSV were examined by assessing the proliferation and differentiation properties of MSCs.
hUC-MSCs were isolated from Wharton's jelly, including the smooth muscle layer of the umbilical cord. During subculture, the cells were passaged every 5-6 days using nonhematopoietic stem cell media. To measure the effect of sonic vibration, SSV was applied to these cells continuously for 5 days.
In this study, the morphology of hUC-MSCs was altered to resemble neurons by SSV. Further, the mRNA and protein levels of neuron-specific markers, including MAP2, NF-L, and NeuroD1, increased. In addition, other neural cell markers, such as GFAP and O4, were increased. These results suggest that hUC-MSCs differentiated into neural cells upon SSV nonselectively. In a mechanism study, the ERK level increased in a time-dependent manner upon SSV for 12 h.
The results of this study suggest that SSV caused hUC-MSCs to differentiate into neural cells via ERK activation.
成体干细胞，如脐带间充质干细胞（UC-MSCs），具有分化为多种类型细胞的潜能，包括神经元。研究表明，机械刺激会引起 MSCs 的反应，具体来说，低强度和高强度亚声振动（SSV）已被证明有利于伤口愈合。在这项研究中，通过评估 MSC 的增殖和分化特性来检查 SSV 的作用。
hUC-MSCs 从脐带华通氏胶中分离出来，包括脐带的平滑肌层。在亚培养过程中，使用非造血干细胞培养基每隔 5-6 天传代一次。为了测量声振动的影响，将 SSV 连续施加到这些细胞 5 天。
在这项研究中，SSV 将 hUC-MSCs 的形态改变为神经元样。此外，神经元特异性标志物的 mRNA 和蛋白水平，包括 MAP2、NF-L 和 NeuroD1，增加。此外，还增加了其他神经细胞标志物，如 GFAP 和 O4。这些结果表明，hUC-MSCs 在 SSV 作用下非选择性地分化为神经细胞。在机制研究中，ERK 水平在 SSV 作用 12 小时内呈时间依赖性增加。
这项研究的结果表明，SSV 通过 ERK 激活引起 hUC-MSCs 分化为神经细胞。
