Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
Int J Mol Sci. 2022 Jan 29;23(3):1582. doi: 10.3390/ijms23031582.
To investigate the effect of eupatilin in asthma treatment, we evaluated its therapeutic effect and related signal transduction in OVA-induced asthmatic mice and LPS-stimulated RAW264.7 cells. The BALF was tested for changes in lung inflammatory cells. Th2 cytokines in the BALF and OVA-IgE in the serum were measured by ELISA. H&E and PAS staining were used to evaluate histopathological changes in mouse lungs. The key proteins NF-κB, MAPK, and Nrf2 in lung tissues were quantitatively analyzed by Western blotting. Finally, we evaluated the effect of eupatilin on cytokines and related protein expression in LPS-stimulated RAW 264.7 cells in vitro. In OVA-induced asthmatic mice, eupatilin reduced the numbers of inflammatory cells, especially neutrophils and eosinophils. Eupatilin also decreased the levels of IL-5, IL-13 in the BALF and OVA-IgE in the serum. Furthermore, eupatilin inhibited the activation of NF-κB and MAPK pathways and increased the expression of Nrf2 in OVA-induced asthmatic mice. In vitro, eupatilin significantly reduced LPS-stimulated NO, IL-6, and ROS production. Additionally, the NF-κB, MAPK, and Nrf2 protein expression in LPS-stimulated RAW264.7 cells was consistent with that in OVA-induced asthmatic lung tissues. In summary, eupatilin attenuated OVA-induced asthma by regulating NF-κB, MAPK, and Nrf2 signaling pathways. These results suggest the utility of eupatilin as an anti-inflammatory drug for asthma treatment.
为了研究芹菜素在哮喘治疗中的作用，我们评估了其在卵清蛋白（OVA）诱导的哮喘小鼠和脂多糖（LPS）刺激的 RAW264.7 细胞中的治疗效果和相关信号转导。通过酶联免疫吸附试验（ELISA）检测支气管肺泡灌洗液（BALF）中肺炎症细胞的变化。通过 ELISA 测定 BALF 中的 Th2 细胞因子和血清中的 OVA-IgE。通过 H&E 和 PAS 染色评估小鼠肺部的组织病理学变化。通过 Western blot 定量分析肺组织中 NF-κB、MAPK 和 Nrf2 等关键蛋白。最后，我们评估了芹菜素对 LPS 刺激的 RAW264.7 细胞中细胞因子和相关蛋白表达的体外影响。在 OVA 诱导的哮喘小鼠中，芹菜素减少了炎症细胞的数量，特别是中性粒细胞和嗜酸性粒细胞。芹菜素还降低了 BALF 中的 IL-5、IL-13 和血清中的 OVA-IgE 水平。此外，芹菜素抑制了 OVA 诱导的哮喘小鼠中 NF-κB 和 MAPK 通路的激活，并增加了 Nrf2 的表达。在体外，芹菜素显著减少了 LPS 刺激的 NO、IL-6 和 ROS 的产生。此外，LPS 刺激的 RAW264.7 细胞中 NF-κB、MAPK 和 Nrf2 蛋白表达与 OVA 诱导的哮喘肺组织中的表达一致。综上所述，芹菜素通过调节 NF-κB、MAPK 和 Nrf2 信号通路减轻 OVA 诱导的哮喘。这些结果表明芹菜素可作为治疗哮喘的抗炎药物。
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