State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, China.
College of Agriculture, Guangdong Ocean University, Zhanjiang 524088, China.
Int J Mol Sci. 2022 Jan 30;23(3):1624. doi: 10.3390/ijms23031624.
Salinity is one of the most common unfavorable environmental conditions that limits plant growth and development, ultimately reducing crop productivity. To investigate the underlying molecular mechanism involved in the salinity response in rice, we initially screened 238 rice cultivars after salt treatment at the seedling stage and identified two highly salt-tolerant cultivars determined by the relative damage rate parameter. The majority of cultivars (94.1%) were ranked as salt-sensitive and highly salt-sensitive. Transcriptome profiling was completed in highly salt-tolerant, moderately salt-tolerant, and salt-sensitive under water and salinity treatments at the seedling stage. Principal component analysis displayed a clear distinction among the three cultivars under control and salinity stress conditions. Several starch and sucrose metabolism-related genes were induced after salt treatment in all genotypes at the seedling stage. The results from the present study enable the identification of the ascorbate glutathione pathway, potentially participating in the process of plant response to salinity in the early growth stage. Our findings also highlight the significance of high-affinity K uptake transporters (HAKs) and high-affinity K transporters (HKTs) during salt stress responses in rice seedlings. Collectively, the cultivar-specific stress-responsive genes and pathways identified in the present study act as a useful resource for researchers interested in plant responses to salinity at the seedling stage.
盐度是限制植物生长和发育的最常见不利环境条件之一，最终会降低作物的生产力。为了研究水稻盐响应的潜在分子机制，我们在幼苗期用盐处理后初步筛选了 238 个水稻品种，根据相对损伤率参数确定了两个高度耐盐的品种。大多数品种（94.1%）被归类为盐敏感型和高度盐敏感型。在幼苗期水和盐处理下，对高度耐盐、中度耐盐和盐敏感的品种进行了转录组谱分析。主成分分析显示，在对照和盐胁迫条件下，三种品种之间有明显的区别。在所有基因型的幼苗期盐处理后，几个淀粉和蔗糖代谢相关基因被诱导。本研究的结果可以确定抗坏血酸-谷胱甘肽途径，该途径可能参与了植物在早期生长阶段对盐胁迫的反应过程。我们的研究结果还强调了高亲和钾摄取转运体（HAKs）和高亲和钾转运体（HKTs）在水稻幼苗盐胁迫反应中的重要性。总之，本研究中鉴定的品种特异性应激响应基因和途径为研究人员提供了一个有用的资源，有助于了解植物在幼苗期对盐胁迫的反应。
