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渗透处理和 NaCl 处理对不结球白菜离子转运蛋白编码基因表达的影响比较。

Comparison between the impact of osmotic and NaCl treatments on the expression of genes coding for ion transporters in Oryza glaberrima Steud.

机构信息

Groupe de Recherche en Physiologie végétale - Earth and Life Institute-Agronomy (ELIA) - Université catholique de Louvain, Louvain-la-Neuve, Belgium.

Laboratoire de Physiologie végétale et d'Etude des Stress environnementaux, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, Cotonou, République du Bénin.

出版信息

PLoS One. 2023 Nov 15;18(11):e0290752. doi: 10.1371/journal.pone.0290752. eCollection 2023.

Abstract

We analyzed the expression of genes coding for Na+ transporters (OsHKT1.5, OsHKT1.1, OsSOS1, OsSOS2, OsNHX1, OsNHX2), Cl- transporter (OsNRT1, OsCLC, OsCCC1) and gene coding for the transcription factor DREB (OsDREB2) involved in response to desiccation in two cultivars of O. glaberrrima differing in salt-resistance (salt-tolerant cultivar (TOG5307) and salt-sensitive (TOG 5949)) exposed to NaCl, PEG or both agents present simultaneously. Seedlings were grown in iso-osmotic nutrient solution (Ψs = -0.47±0.02 MPa) containing PEG 6,000 12.9% (water stress), NaCl 75 mM (salt stress) and PEG 6.4% + NaCl 37.5 mM (MIX-treatment) during 1 and 7 days. Plants were analyzed for gene expression, mineral nutrients, and photosynthetic-related parameters. Na+ and Cl- accumulations in salt-treated plants were lower in roots and shoots of TOG5307 comparatively to TOG5949 while water content decreased in TOG5307. TOG5307 exhibited tolerance to water stress and maintained higher net photosynthesis and water use efficiency than TOG5949 in response to all treatments, but was less efficient for osmotic adjustment. Dehydration tolerance of TOG5307 involves a higher OsDREB2 expression. TOG5307 also exhibited a higher OsSOS1, OsSOS2, OsNHX1 and OsNHX2 expression than TOG5949 in response to salinity. OsHKT1.5 was slightly induced in the shoot. OsHKT1.1 was recorded in the shoots but remained undetectable in the roots. Chloride and sodium accumulations were strongly reduced in the shoots when PEG was present. Salinity resistance in Oryza glaberrima implies tolerance to dehydration as well as complementary strategies of Na+ exclusion through the SOS system and Na+ tolerance through vacuolar sequestration.

摘要

我们分析了编码 Na+转运蛋白(OsHKT1.5、OsHKT1.1、OsSOS1、OsSOS2、OsNHX1、OsNHX2)、Cl-转运蛋白(OsNRT1、OsCLC、OsCCC1)和参与响应脱水的转录因子 DREB(OsDREB2)的基因在两个耐盐性不同的 O. glaberrrima 品种(耐盐品种(TOG5307)和盐敏感品种(TOG 5949))中的表达,这些品种暴露于 NaCl、PEG 或同时存在这两种试剂中。幼苗在含有 PEG 6000 的等渗营养液(Ψs = -0.47±0.02 MPa)中生长,PEG 含量为 12.9%(水分胁迫)、NaCl 75 mM(盐胁迫)和 PEG 6.4%+NaCl 37.5 mM(MIX 处理),处理 1 天和 7 天。对植物进行基因表达、矿质养分和光合作用相关参数分析。与 TOG5949 相比,TOG5307 的根和茎中的 Na+和 Cl-积累在盐处理的植物中较低,而 TOG5307 的含水量下降。TOG5307 对水分胁迫表现出耐受性,并在所有处理中保持比 TOG5949 更高的净光合作用和水分利用效率,但在渗透调节方面效率较低。TOG5307 的耐旱性涉及更高的 OsDREB2 表达。TOG5307 对盐胁迫的反应还表现出比 TOG5949 更高的 OsSOS1、OsSOS2、OsNHX1 和 OsNHX2 表达。OsHKT1.5 在地上部略有诱导。OsHKT1.1 在地上部被记录,但在根部仍无法检测到。PEG 存在时,地上部的氯和钠积累明显减少。Oryza glaberrima 的耐盐性意味着它既耐受脱水,又通过 SOS 系统排除 Na+和液泡隔离 Na+来耐受盐胁迫。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a82/10650995/f4b6cc9dcb13/pone.0290752.g001.jpg

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