Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Plant Genetics, Gembloux Agro-Bio Tech, University of Liège, 5030 Gembloux, Belgium.
Int J Mol Sci. 2022 Feb 1;23(3):1708. doi: 10.3390/ijms23031708.
Previously, the complexity of folate accumulation in the early stages of maize kernel development has been reported, but the mechanisms of folate accumulation are unclear. Two maize inbred lines, DAN3130 and JI63, with different patterns of folate accumulation and different total folate contents in mature kernels were used to investigate the transcriptional regulation of folate metabolism during late stages of kernel formation by comparative transcriptome analysis. The folate accumulation during DAP 24 to mature kernels could be controlled by circumjacent pathways of folate biosynthesis, such as pyruvate metabolism, glutamate metabolism, and serine/glycine metabolism. In addition, the folate variation between these two inbred lines was related to those genes among folate metabolism, such as genes in the pteridine branch, para-aminobenzoate branch, serine/tetrahydrofolate (THF)/5-methyltetrahydrofolate cycle, and the conversion of THF monoglutamate to THF polyglutamate. The findings provided insight into folate accumulation mechanisms during maize kernel formation to promote folate biofortification.
先前已经报道了玉米籽粒发育早期叶酸积累的复杂性，但叶酸积累的机制尚不清楚。本研究使用两个具有不同叶酸积累模式和成熟籽粒中总叶酸含量的玉米自交系 DAN3130 和 JI63，通过比较转录组分析研究了籽粒形成后期叶酸代谢的转录调控。从 DAP24 到成熟籽粒的叶酸积累可以通过叶酸生物合成的周边途径来控制，如丙酮酸代谢、谷氨酸代谢和丝氨酸/甘氨酸代谢。此外，这两个自交系之间的叶酸变化与叶酸代谢中的那些基因有关，如蝶啶分支、对氨基苯甲酸分支、丝氨酸/四氢叶酸（THF）/5-甲基四氢叶酸循环以及 THF 单谷氨酸向 THF 多谷氨酸的转化。这些发现为促进叶酸生物强化提供了在玉米籽粒形成过程中叶酸积累机制的深入了解。
