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干旱响应 NAC 转录因子被 识别,与 相互作用以增强拟南芥的耐旱性。

Drought-Responsive NAC Transcription Factor Is Recognized by , Interacts with to Enhance Drought Tolerance in Arabidopsis.

机构信息

Beijing Key Laboratory of Ornamental Germplasm Innovation and Molecular Breeding, China National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China.

Beijing Key Laboratory of Greening Plant Breeding, Beijing Institute of Landscape Architecture, Beijing 100201, China.

出版信息

Int J Mol Sci. 2022 Feb 3;23(3):1755. doi: 10.3390/ijms23031755.

Abstract

, a key transcription factor that may respond to drought stress in 'Old Blush', was selected in our previous study. In the present study, we found that is localized in the nucleus and is a transcriptional activator. expression could be significantly induced by drought, low temperature, salt as well as abscisic acid (ABA) treatment. Analysis of the promoter revealed that multiple abiotic stress and hormone response elements were located in the promoter region. The promoter could respond to drought, low temperature, salt and ABA treatments to activate GUS gene expression. Overexpressing in Arabidopsis thaliana enhanced sensitivity to ABA and tolerance to drought stress. Silencing of by virus-induced gene silencing (VIGS) in rose leaves significantly reduced leaf water loss tolerance and leaf extension capacity. Physical interaction of with was shown by means of the yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays. was demonstrated to be able to bind to the promoter of by means of the yeast one-hybrid (Y1H) assay. These results provide new insights into the regulatory network of response to drought stress in roses.

摘要

在我们之前的研究中,选择了一个关键的转录因子,可能对“老红晕”(一种玫瑰品种)的干旱胁迫做出反应。在本研究中,我们发现 定位在细胞核中,是一个转录激活因子。干旱、低温、盐和脱落酸(ABA)处理均可显著诱导 表达。启动子分析表明,多个非生物胁迫和激素响应元件位于启动子区域。启动子可以响应干旱、低温、盐和 ABA 处理,激活 GUS 基因表达。在拟南芥中过表达 增强了对 ABA 的敏感性和对干旱胁迫的耐受性。通过病毒诱导的基因沉默(VIGS)在玫瑰叶片中沉默 显著降低了叶片水分损失的耐受性和叶片伸展能力。酵母双杂交(Y2H)和双分子荧光互补(BiFC)实验表明 与 之间存在物理相互作用。酵母单杂交(Y1H)实验表明 能够与 启动子结合。这些结果为玫瑰响应干旱胁迫的 调控网络提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c6/8915184/a31c47e55367/ijms-23-01755-g001.jpg

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