Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China.
Lushan Botanical Garden, Chinese Academy of Sciences, Jiujiang 332900, China.
Int J Mol Sci. 2021 Nov 2;22(21):11897. doi: 10.3390/ijms222111897.
Kiwifruit (Actinidia chinensis Planch) is suitable for neutral acid soil. However, soil salinization is increasing in kiwifruit production areas, which has adverse effects on the growth and development of plants, leading to declining yields and quality. Therefore, analyzing the salt tolerance regulation mechanism can provide a theoretical basis for the industrial application and germplasm improvement of kiwifruit. We identified 120 NAC members and divided them into 13 subfamilies according to phylogenetic analysis. Subsequently, we conducted a comprehensive and systematic analysis based on the conserved motifs, key amino acid residues in the NAC domain, expression patterns, and protein interaction network predictions and screened the candidate gene . In order to study its function, we adopted the method of heterologous expression in . Compared with the control, the overexpression plants had higher osmotic adjustment ability and improved antioxidant defense mechanism. These results suggest that plays a positive role in the salt tolerance regulation mechanism in kiwifruit.
猕猴桃（Actinidia chinensis Planch）适合中性偏酸性土壤。然而，猕猴桃产区的土壤盐碱化日益严重，这对植物的生长发育产生了不利影响，导致产量和品质下降。因此，分析猕猴桃的耐盐调控机制可为猕猴桃的产业化应用和种质改良提供理论依据。我们鉴定了 120 个 NAC 成员，并根据系统发育分析将它们分为 13 个亚家族。随后，我们基于保守基序、NAC 结构域中的关键氨基酸残基、表达模式以及蛋白质相互作用网络预测进行了全面而系统的分析，并筛选出候选基因。为了研究其功能，我们采用了在 中的异源表达方法。与对照相比，过表达植株具有更高的渗透调节能力和改善的抗氧化防御机制。这些结果表明 在猕猴桃的耐盐调控机制中发挥着积极作用。
