Laboratorio de Biología Molecular y Biotecnología Vegetal, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 8320000, Chile.
Centro de Biología Molecular Vegetal, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago 7750000, Chile.
Int J Mol Sci. 2022 Feb 5;23(3):1827. doi: 10.3390/ijms23031827.
Agronomic breeding practices for grapevines ( L.) include the application of growth regulators in the field. Brassinosteroids (BRs) are a family of sterol-derived plant hormones that regulate several physiological processes and responses to biotic and abiotic stress. In grapevine berries, the production of biologically active BRs, castasterone and 6-deoxocastasterone, has been reported. In this work, key BR genes were identified, and their expression profiles were determined in grapevine. Bioinformatic homology analyses of the Arabidopsis genome found 14 genes associated with biosynthetic, perception and signaling pathways, suggesting a partial conservation of these pathways between the two species. The tissue- and development-specific expression profiles of these genes were determined by qRT-PCR in nine different grapevine tissues. Using UHPLC-MS/MS, 10 different BR compounds were pinpointed and quantified in 20 different tissues, each presenting specific accumulation patterns. Although, in general, the expression profile of the biosynthesis pathway genes of BRs did not directly correlate with the accumulation of metabolites, this could reflect the complexity of the BR biosynthesis pathway and its regulation. The development of this work thus generates a contribution to our knowledge about the presence, and diversity of BRs in grapevines.
园艺学培育葡萄（L.）的实践包括在田间应用生长调节剂。油菜素内酯（BRs）是一类固醇衍生的植物激素，调节多种生理过程和对生物及非生物胁迫的响应。在葡萄浆果中，已经报道了具有生物活性的 BRs，即油菜素内酯和 6-脱甲油菜素内酯的产生。在这项工作中，鉴定了关键的 BR 基因，并确定了它们在葡萄中的表达谱。对拟南芥基因组的生物信息学同源性分析发现了 14 个与生物合成、感知和信号转导途径相关的基因，这表明这两个物种之间这些途径存在部分保守性。通过 qRT-PCR 在 9 种不同的葡萄组织中确定了这些基因的组织和发育特异性表达谱。使用 UHPLC-MS/MS，在 20 种不同的组织中鉴定和定量了 10 种不同的 BR 化合物，每种化合物都呈现出特定的积累模式。尽管通常情况下，BR 生物合成途径基因的表达谱与代谢物的积累没有直接相关性，但这可能反映了 BR 生物合成途径及其调控的复杂性。因此，这项工作的开展为我们了解葡萄中 BR 的存在和多样性做出了贡献。
