Hubbard J D, Janssen H F
Circ Shock. 1986;18(3):179-92.
This study was designed to examine glucocorticoid effects on increases in vascular permeability caused by endotoxic shock in dogs anesthetized with pentobarbital sodium (30 mg/kg). Methylprednisolone sodium succinate was administered IV in two doses (30 mg/kg each) before Escherichia coli endotoxin was administered (0.5 mg/kg). Samples of serum and lymph from the left thoracic duct were collected for measurement of total protein and separation by polyacrylamide gel electrophoresis. The same four protein electrophoretic fractions with molecular weights (M.W.) ranging from 60,000 to 1,000,000 were consistently chosen for analysis. Methylprednisolone treatment given prior to endotoxin administration resulted in an attenuation of the early increase in total protein flux and lymph to plasma protein (L/P) ratio and prevented significant increases in the permeability surface area product observed in the group given endotoxin alone. Endotoxin administration alone resulted in significant increases in the permeability-surface area product and L/P ratio for all four electrophoretic fractions. Pretreatment with glucocorticoid partially attenuated the increase in the L/P ratio for only those fractions of 100,000 M.W. or less. These results suggest MP provides partial but not complete protection from increases in vascular permeability during endotoxic shock.
本研究旨在考察糖皮质激素对戊巴比妥钠（30毫克/千克）麻醉的犬内毒素休克所致血管通透性增加的影响。在给予大肠杆菌内毒素（0.5毫克/千克）之前，静脉注射两次甲泼尼龙琥珀酸钠（每次30毫克/千克）。采集血清样本和左胸导管的淋巴液，用于测定总蛋白，并通过聚丙烯酰胺凝胶电泳进行分离。始终选择分子量（M.W.）在60,000至1,000,000之间的相同四种蛋白质电泳组分进行分析。在内毒素给药前给予甲泼尼龙治疗，可使总蛋白通量和淋巴与血浆蛋白（L/P）比值的早期增加减弱，并防止在仅给予内毒素的组中观察到的通透表面积乘积显著增加。单独给予内毒素可使所有四种电泳组分的通透表面积乘积和L/P比值显著增加。糖皮质激素预处理仅部分减弱了分子量为100,000或更小的组分的L/P比值增加。这些结果表明，甲泼尼龙对内毒素休克期间血管通透性增加提供部分而非完全保护。
