Department of Animal Science and Biotechnology, College of Ecology and Environmental Science, Kyungpook National University, Sangju, Republic of Korea.
Laboratory of Veterinary Internal Medicine, BK21 PLUS Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Vector Borne Zoonotic Dis. 2022 Mar;22(3):178-183. doi: 10.1089/vbz.2021.0060. Epub 2022 Feb 14.
Babesiosis is a tick-borne disease caused by intraerythrocytic protozoa belonging to the genus that is primarily transmitted through Ixodid ticks and is highly pathogenic to ruminants, horses, pigs, dogs, cats, and in some cases, even humans. is considered an increasing threat to animal and human health. In this study we report infection in Korean water deer (). Blood samples of 77 wild animals (62 Korean water deer and 15 Korean raccoon dogs []) were analyzed to detect infection. First, PCR was performed to investigate infection using the partial 18S rRNA gene. Among the 77 wild animals examined, 11 Korean water deer were positive for infection as per the results of sequence analysis. Next, these 11 samples were reanalyzed by PCR using newly designed primers to differentiate between and . Finally, only one Korean water deer sample was found to be positive for . The nucleotide sequence of this protozoan showed 99.6 - 99.7% identity to those of reported previously. Phylogenetic analysis revealed that the sequence belonged to the clade and diverged from the clade. Three nucleotides at positions 631, 663, and 1637 were analyzed to differentiate between and . The nucleotides at positions 631 and 1637 were identical to those of , whereas that at position 663 was different. The sequence detected in Korean water deer also exhibited differences at other four positions (669, 704, 711, and 1661) compared with other sequences. This study indicates that Korean water deer serve as a reservoir for . In addition, the primers designed in this study can be used to detect . Future studies should explore the tick vectors that transmit and its pathogenicity. This study was approved by the Institutional Committee of Graduate Studies and Research at Jeonbuk National University, ROK (IACUC decision no.: CBU 2014-00026).
梨形虫病是一种由红细胞内原生动物引起的蜱传疾病，属于 ，主要通过硬蜱传播，对反刍动物、马、猪、狗、猫具有高度致病性，在某些情况下，甚至对人类也具有致病性。 被认为是对动物和人类健康的日益威胁。在本研究中，我们报告了在韩国水鹿（）中感染 。对 77 只野生动物（62 只韩国水鹿和 15 只韩国浣熊犬）的血液样本进行了分析，以检测 感染。首先，使用部分 18S rRNA 基因进行 PCR 检测 感染。在所检查的 77 只野生动物中，根据序列分析结果，有 11 只韩国水鹿呈感染阳性。接下来，使用新设计的引物对这 11 个样本进行了重新分析，以区分 和 。最后，只有一只韩国水鹿样本被检测到为 阳性。该原生动物的核苷酸序列与先前报道的 显示出 99.6-99.7%的同一性。系统发育分析显示，该序列属于 分支，并与 分支分化。对位置 631、663 和 1637 的三个核苷酸进行分析，以区分 和 。位置 631 和 1637 的核苷酸与 相同，而位置 663 的核苷酸则不同。在韩国水鹿中检测到的序列与其他 序列在其他四个位置（669、704、711 和 1661）也存在差异。本研究表明，韩国水鹿是 的储存宿主。此外，本研究中设计的引物可用于检测 。未来的研究应探索传播 和其致病性的蜱媒。本研究得到了韩国全州国立大学研究生院和研究机构的机构委员会的批准（IACUC 决定号：CBU 2014-00026）。
