College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Wusi Road, Baoding, 071002, People's Republic of China.
Anal Bioanal Chem. 2022 Mar;414(8):2597-2606. doi: 10.1007/s00216-022-03895-x. Epub 2022 Feb 15.
Although some ratiometric fluorescent sensors have been reported to detect tetracyclines, most of ratiometric fluorescent sensors were established based on europium ion with a narrow linear range. In this work, a ratiometric fluorescent sensor for tetracyclines detection was established based on the dual-emission lanthanum-doped carbon dots (La-CDs) as probes combining with the characteristic pH-response of tetracyclines. The fluorescence intensity of tetracyclines will be enhanced in high pH, and the emission peak of tetracyclines overlapped with the peak of probes. The superposition effect of tetracyclines and probes at 515 nm greatly improved the sensitivity of the ratiometric fluorescent sensor and widened the detection range, and linear ranges for oxytetracycline (OTC) and tetracycline (TC) were respectively 0.00-805.20 μM and 0.00-1039.50 μM. Moreover, the preparation procedure of the La-CDs was simple and time saving and the coupling agent was not required. A comparison of La-CDs with undoped carbon dots (un-CDs) showed that the optical performance and sensing performance of La-CDs were improved. In addition, a portable paper sensor with La-CDs as probes was preliminarily explored in this work, and the sensor has been applied to detect OTC and TC in pork and fish with satisfactory results.
尽管已经有一些比率型荧光传感器被报道用于检测四环素类药物，但大多数比率型荧光传感器都是基于镧离子建立的，其线性范围较窄。在这项工作中，建立了一种基于双发射镧掺杂碳点（La-CDs）作为探针的比率型荧光传感器，结合四环素类药物的特征 pH 响应来检测四环素类药物。在高 pH 值下，四环素类药物的荧光强度会增强，并且四环素类药物的发射峰与探针的发射峰重叠。在 515nm 处，四环素类药物和探针的叠加效应对比率型荧光传感器的灵敏度有很大的提高，并且拓宽了检测范围，氧四环素（OTC）和四环素（TC）的线性范围分别为 0.00-805.20 μM 和 0.00-1039.50 μM。此外，La-CDs 的制备过程简单、省时，且不需要偶联剂。La-CDs 与未掺杂的碳点（un-CDs）的比较表明，La-CDs 的光学性能和传感性能得到了改善。此外，本工作初步探索了以 La-CDs 为探针的便携式纸质传感器，并将其应用于猪肉和鱼肉中 OTC 和 TC 的检测，结果令人满意。
