Zhao Tengzhi, Wang Zhaofeng, Zhang Runke, Xu Hui, Wang Hua, Yu Ying
School of Life Sciences, Huzhou University, Zhejiang, 313000, P.R. China.
School of Chemistry and Environmental Engineering, China University of Mining and Technology (Beijing), Beijing, 100083, P.R. China.
J Fluoresc. 2025 Sep 9. doi: 10.1007/s10895-025-04531-4.
The antibiotic contamination in aquatic environments, particularly in aquaculture systems, poses substantial risks to ecological balance and human health. To address this issue, we engineered a novel ratiometric fluorescent probe utilizing dual-emission carbon dots (D-CDs) synthesized from sustainable biomass carrot and nitrogen-rich precursors (melamine and o-phenylenediamine) through an efficient one-pot hydrothermal approach. The D-CDs exhibited dual emission peaks at 425nm and 540 nm under 370nm excitation. The fluorescence intensity ratio (F/F) demonstrated linear response toward tetracycline (TC) and oxytetracycline (OTC), with wider linear detection ranges of 0.027-133.3 µM for TC and 0.027-233.3 µM for OTC, respectively. Low detection limits (17.7 nM for TC and 22.04 nM for OTC) were also achieved. Mechanistic studies revealed that quenching resulted from static quenching and inner filter effects. The probe showed exceptional stability under high ionic strength and long storage conditions. Real-sample monitoring in lake water and aquaculture samples yielded recovery rates of 90.4-103.5% with RSD < 6.88%, which was validated by high-performance liquid chromatography (HPLC), demonstrating the promising potential of this probe.
水生环境中的抗生素污染，尤其是水产养殖系统中的抗生素污染，对生态平衡和人类健康构成了重大风险。为了解决这个问题，我们设计了一种新型的比率荧光探针，它利用可持续生物质胡萝卜与富氮前体（三聚氰胺和邻苯二胺）通过高效的一锅水热法合成的双发射碳点（D-CDs）。在370nm激发下，D-CDs在425nm和540nm处呈现双发射峰。荧光强度比（F/F）对四环素（TC）和土霉素（OTC）表现出线性响应，TC的线性检测范围为0.027-133.3µM，OTC的线性检测范围为0.027-233.3µM。同时也实现了低检测限（TC为17.7 nM，OTC为22.04 nM）。机理研究表明，猝灭是由静态猝灭和内滤效应引起的。该探针在高离子强度和长时间储存条件下表现出优异的稳定性。湖水和水产养殖样品的实际样品监测回收率为90.4-103.5%，相对标准偏差<6.88%，经高效液相色谱（HPLC）验证，证明了该探针具有广阔的应用前景。
