细丝蛋白在心肌中的分离与定位

Isolation and localization of filamin in heart muscle.

作者信息

Koteliansky V E, Glukhova M A, Gneushev G N, Samuel J L, Rappaport L

出版信息

Eur J Biochem. 1986 May 2;156(3):619-23. doi: 10.1111/j.1432-1033.1986.tb09622.x.

DOI:10.1111/j.1432-1033.1986.tb09622.x

Abstract

High-molecular-mass protein was isolated from chicken heart muscle. The apparent molecular mass of a single polypeptide chain is similar to that of chicken gizzard filamin: 250-270 kDa. The protein interacts with antibodies against chicken gizzard filamin and induces F-actin gelation in a concentration-dependent manner. Immunofluorescent staining of cardiomyocytes and chicken heart sections with antifilamin antibody demonstrates two types of filamin localization: filamin was located on the sarcomere border in the periphery of the Z-disk; filamin was found in intercalated disks between cardiomyocytes.

摘要

从鸡心肌中分离出高分子量蛋白质。单个多肽链的表观分子量与鸡胗细丝蛋白相似：250 - 270 kDa。该蛋白质与抗鸡胗细丝蛋白抗体相互作用，并以浓度依赖的方式诱导F - 肌动蛋白凝胶化。用抗细丝蛋白抗体对心肌细胞和鸡心脏切片进行免疫荧光染色显示出两种细丝蛋白定位类型：细丝蛋白位于Z盘周边的肌节边界；细丝蛋白存在于心肌细胞之间的闰盘中。