Diabetes, Endocrinology, and Obesity Branch, Intramural Research Program, National Institute of Diabetes, Bethesda, MD, USA.
Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.
Methods Mol Biol. 2022;2448:83-96. doi: 10.1007/978-1-0716-2087-8_5.
An appealing strategy for treatment of metabolic disease in humans is activation of brown adipose tissue (BAT), a thermogenic organ best visualized through F-FDG PET/CT. BAT has been activated to varying degrees by mild cold exposure. However, this approach can cause undesirable stress, and there remains no consensus protocol. Here, we describe standardized methods for both acute and chronic activation of BAT using the orally administered β3-adrenergic receptor (AR) agonist, mirabegron. Acute pharmacological stimulation has enabled quantification of whole-body BAT volume and metabolic activity using PET/CT imaging, and chronic stimulation increases these properties of BAT over time.
激活棕色脂肪组织（BAT）是治疗人类代谢疾病的一种有吸引力的策略，这种产热器官可以通过 F-FDG PET/CT 很好地观察到。适度的寒冷暴露可以在不同程度上激活 BAT。然而，这种方法会引起不必要的应激，而且目前还没有共识的方案。在这里，我们描述了使用口服β3 肾上腺素能受体（AR）激动剂米拉贝隆来急性和慢性激活 BAT 的标准化方法。急性药物刺激使我们能够使用 PET/CT 成像来定量全身 BAT 体积和代谢活性，而慢性刺激会随着时间的推移增加 BAT 的这些特性。
