Sherwood R A, Brent L, Rayfield L S
Eur J Immunol. 1986 May;16(5):569-74. doi: 10.1002/eji.1830160519.
Presentation of alloantigens by host cells has been examined in vivo by means of a murine cell transfer system. Primary (1 degree) hosts were activated by the i.p. administration of allogeneic spleen cells and their spleen or peritoneal cells were transferred into syngeneic secondary (2 degrees) hosts 3 days later. Sensitization of 2 degrees hosts was assessed by their ability to reject donor strain skin grafts prematurely. The transferred cells were routinely depleted of T lymphocytes. We show that (a) 5 X 10(7) spleen and 3 X 10(6) peritoneal cells consistently caused marked accelerated graft rejection; (b) this effect was antigen specific and observable in all strain combinations studied; (c) it was caused by the active sensitization of 2 degrees hosts, but not by contaminating donor strain cells; (d) the cells involved were plastic adherent and viability was not a requirement; and (e) both class I and II, but not minor, histocompatibility antigens played a role in this model. We conclude that presentation of alloantigens by host antigen-presenting cells can be a potent route of allosensitization.
通过小鼠细胞转移系统在体内检测了宿主细胞对抗同种异体抗原的呈递。通过腹腔注射同种异体脾细胞激活初级（1°）宿主，3天后将其脾细胞或腹腔细胞转移至同基因二级（2°）宿主。通过二级宿主过早排斥供体品系皮肤移植物的能力来评估其致敏情况。常规去除转移细胞中的T淋巴细胞。我们发现：（a）5×10⁷个脾细胞和3×10⁶个腹腔细胞始终会导致明显加速的移植物排斥反应；（b）这种效应具有抗原特异性，在所研究的所有品系组合中均可观察到；（c）它是由二级宿主的主动致敏引起的，而非供体品系细胞的污染；（d）所涉及的细胞具有贴壁可塑性，且活力并非必需条件；（e）在该模型中，I类和II类组织相容性抗原而非次要组织相容性抗原发挥了作用。我们得出结论，宿主抗原呈递细胞对抗同种异体抗原的呈递可能是同种异体致敏的有效途径。
