Opt Lett. 2022 Feb 15;47(4):814-817. doi: 10.1364/OL.451267.
In this Letter, we present a single-shot 3D-resolved structured illumination microscopy (SIM) based on a digital micromirror device (DMD), a galvanometric mirror, and the HiLo algorithm. During imaging, the DMD rapidly generates sinusoidal and plane illuminations in the focal region. By synchronizing the DMD with a galvanometric scanner and exploiting the unique data readout process of the camera, the emissions from the specimen under two different illuminations, i.e., structured and uniform illumination, are projected to different regions on a camera, achieving high-resolution single-exposure optical sectioning at the camera's limiting speed, i.e., 200 Hz, without sacrificing the resolution. A model has been developed to guide the design and optimization of the optical system. Imaging experiments on pollen and mouse kidney samples have been performed to verify the predicted performance. The results show that the single-shot SIM with the HiLo algorithm achieves comparable resolution to the standard two-shot HiLo method with a twofold speed enhancement, which may find important applications in biophotonics, e.g., visualizing high-speed biological events in vivo.
在这封信件中，我们提出了一种基于数字微镜器件 (DMD)、振镜和 HiLo 算法的单次 3D 分辨结构光照明显微镜 (SIM)。在成像过程中，DMD 可在焦域内快速产生正弦和平面照明。通过将 DMD 与振镜同步，并利用相机独特的数据读出过程，来自标本的两个不同照明下的发射，即结构和均匀照明，被投影到相机上的不同区域，从而在相机的限制速度（即 200 Hz）下实现高分辨率的单次曝光光学切片，而不会牺牲分辨率。我们已经建立了一个模型来指导光学系统的设计和优化。已经进行了花粉和小鼠肾脏样本的成像实验，以验证预测的性能。结果表明，HiLo 算法的单次 SIM 可实现与标准两拍 HiLo 方法相当的分辨率，速度提高了两倍，这在生物光子学等领域可能有重要应用，例如在体内可视化高速生物事件。
