Opt Lett. 2021 Jul 15;46(14):3424-3427. doi: 10.1364/OL.428562.
In this Letter, we present the modeling, design, and characterization of a light sheet-based structured light illumination (SLI) light field microscopy (LFM) system for fast 3D imaging, where a digital micromirror device is employed to rapidly generate designed sinusoidal patterns in the imaging field. Specifically, we sequentially obtain uniformly illuminated and structured light field images, followed by post-processing with a new, to the best of our knowledge, algorithm that combines the deconvolution and HiLo algorithms. This enables fast volumetric imaging with improved optical cross-sectioning capability at a speed of 50 volumes per second over an imaging field of 250×250×80µ in the , , and axis, respectively. Mathematical models have been derived to explain the performance enhancement due to suppressed background noises. To verify the results, imaging experiments on fluorescence beads, fern spore, and Drosophila brain samples, have been performed. The results indicate that the light sheet-based SLI-LFM presents a fast 3D imaging solution with substantially improved optical cross-sectioning capability in comparison with a standard light sheet-based LFM. The new light field imaging method may find important applications in the field of biophotonics.
在这封信件中，我们展示了一种基于片光的结构光照明（SLI）光场显微镜（LFM）系统的建模、设计和特性，用于快速 3D 成像，其中使用数字微镜器件（DMD）在成像场中快速生成设计的正弦图案。具体来说，我们依次获得均匀照明和结构光场图像，然后使用一种新的、据我们所知的算法进行后处理，该算法结合了反卷积和 HiLo 算法。这使得能够以每秒 50 个体积的速度在成像场中进行快速体积成像，其中 、 、 轴的成像场分别为 250×250×80µ，具有改进的光学横截面能力。已经推导出数学模型来解释由于抑制背景噪声而导致的性能增强。为了验证结果，对荧光珠、蕨类孢子和果蝇大脑样本进行了成像实验。结果表明，与标准基于片光的 LFM 相比，基于片光的 SLI-LFM 呈现出快速 3D 成像解决方案，具有显著提高的光学横截面能力。新的光场成像方法可能在生物光子学领域找到重要的应用。
