Li Siqi, Yu Xiaoling, Li Yuan, Zhang Tian
School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan 430070, People's Republic of China.
Wuhan University of Technology Sanya Science and Education Innovation Park, Sanya 572024, People's Republic of China.
Biomed Phys Eng Express. 2022 Mar 4;8(3). doi: 10.1088/2057-1976/ac5528.
For nerve tissue engineering (NTE), scaffolds with the ability to release drugs under control and support the rapid proliferation of cells are very important for the repair of nerve defects. This study aimed to fabricate a conductive drug-loaded fiber mat by electrospinning and assess its potential as a scaffold for Schwann cells proliferation. The conductive polypyrrole (PPy) was coated on an electrospun poly (D, L-lactide) (PLA) fibrous mat, which was simultaneously embedded with protein-loaded chitosan nanoparticles and ibuprofen as a model small molecule drug. The fibrous mat shows suitable conductivity, mechanical properties, and hydrophilicity for NTE. For drug release and degradation studies, the fibrous mat can achieve sustained release of bovine serum albumin (BSA) and ibuprofen, and the PPy coating can increase the surface wettability and conductivity while slowing down the degradation of the fibrous mat. The application of electrical stimulation (ES) to the fibrous mat can accelerate the release of ibuprofen, but there was no significant effect on the release rate of the protein. The fibrous mat showed no cytotoxicity, and Schwann cells (SCs) can adhere, grow, and proliferate well on mats. At the 120th hour of culture, the relative growth rate of SCs on the conductive drug-loaded fibrous mat reached 198.22 ± 2.34%, which was an increase of 37.93% compared to the SCs on the drug-loaded fibrous mat with ES. The density and elongation of SCs on the conductive drug-loaded fibrous mat were greater than those on the PLA fibrous mat, indicating that the conductive polypyrrole-coated electrospun chitosan nanoparticles/PLA fibrous mat has good potential for application in nerve regeneration.
对于神经组织工程（NTE）而言，具备可控释药能力并能支持细胞快速增殖的支架对于神经缺损修复非常重要。本研究旨在通过静电纺丝制备一种导电载药纤维垫，并评估其作为雪旺细胞增殖支架的潜力。将导电聚吡咯（PPy）涂覆在静电纺丝聚（D，L-丙交酯）（PLA）纤维垫上，该纤维垫同时包埋了载蛋白壳聚糖纳米颗粒和作为模型小分子药物的布洛芬。该纤维垫展现出适用于NTE的导电性、机械性能和亲水性。在药物释放和降解研究中，该纤维垫可实现牛血清白蛋白（BSA）和布洛芬的持续释放，并且PPy涂层可增加表面润湿性和导电性，同时减缓纤维垫的降解。对纤维垫施加电刺激（ES）可加速布洛芬的释放，但对蛋白质的释放速率没有显著影响。该纤维垫无细胞毒性，雪旺细胞（SCs）能够在纤维垫上良好地黏附、生长和增殖。在培养的第120小时，导电载药纤维垫上SCs的相对生长速率达到198.22±2.34%，相较于施加ES的载药纤维垫上的SCs，增长了37.93%。导电载药纤维垫上SCs的密度和伸长率均大于PLA纤维垫上的，这表明导电聚吡咯涂覆的静电纺丝壳聚糖纳米颗粒/PLA纤维垫在神经再生方面具有良好的应用潜力。
