Faculty of Biomaterial and Tissue Engineering, Department of Biomedical Engineering (Center of Excellence), Amirkabir University of Technology, P.O. Box: 15875/4413, Tehran 159163/4311, Iran.
Faculty of Biomaterial and Tissue Engineering, Department of Biomedical Engineering (Center of Excellence), Amirkabir University of Technology, P.O. Box: 15875/4413, Tehran 159163/4311, Iran.
Int J Biol Macromol. 2019 Jan;121:625-632. doi: 10.1016/j.ijbiomac.2018.10.022. Epub 2018 Oct 6.
In this paper, nanofibers containing poly(ε-caprolactone) (PCL), chitosan and polypyrrole (PPy) were fabricated using electrospinning to combine advantages of electrospun nanofibers topography with versatile advantages of chitosan and PPy. Various compositions of the PCL/chitosan/PPy polymeric scaffolds were fabricated by electrospinning and were analyzed for their surface topography, hydrophilicity and bioactivity. The results illustrated that chitosan in the scaffold imposed significant advancement in the hydrophilicity of the scaffold as confirmed by a decrease in contact angle up to 66% (123 ± 2.3 for PCL to 41.37 ± 3.51 for PCL/chitosan). The average diameter of the fibers was within the range of 30-180 nm, which influenced by the concentration of the chitosan as the increase up to 30% in chitosan content decreased fiber diameter from 124 nm to 36 nm. In-vitro studies using PC12 cells revealed that the PCL/chitosan/PPy nanofibrous scaffold supports cell attachment, spreading and revealed significant increase in proliferation up to 356% in comparison to Pure PCL and neurite extension of PC12. The results indicated the PCL/chitosan/PPy nanofibrous scaffolds support the adhesion, spreading and proliferation of the PC12 cells. Therefore, this scaffold could serve as promising neural tissue substitutes.
本文采用静电纺丝技术制备了含有聚己内酯（PCL）、壳聚糖和聚吡咯（PPy）的纳米纤维，将静电纺纳米纤维的拓扑结构优势与壳聚糖和 PPy 的多种优势相结合。通过静电纺丝制备了不同组成的 PCL/壳聚糖/PPy 聚合物支架，并对其表面形貌、亲水性和生物活性进行了分析。结果表明，支架中的壳聚糖显著提高了支架的亲水性，接触角从 PCL 的 123±2.3°降低至 PCL/壳聚糖的 41.37±3.51°，降低了 66%。纤维的平均直径在 30-180nm 范围内，受壳聚糖浓度的影响，壳聚糖含量增加 30%，纤维直径从 124nm 降低至 36nm。用 PC12 细胞进行的体外研究表明，PCL/壳聚糖/PPy 纳米纤维支架支持细胞黏附、铺展，并显示出明显的增殖增加，与纯 PCL 相比增加了 356%，PC12 的神经突延伸也增加。结果表明，PCL/壳聚糖/PPy 纳米纤维支架支持 PC12 细胞的黏附、铺展和增殖。因此，这种支架可以作为有前途的神经组织替代品。
