State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, University of Chinese Academy of Sciences, Beijing, 100049, China.
Guangdong Institute of Intelligence Science and Technology, Hengqin, Zhuhai, 519031, China.
J Neurosci. 2022 Mar 30;42(13):2690-2700. doi: 10.1523/JNEUROSCI.1427-21.2022. Epub 2022 Feb 15.
DRG neurons are classified into distinct types to mediate the somatosensation with different modalities. Recently, transcriptional profilings of DRG neurons by single-cell RNA-sequencing have provided new insights into the neuron typing and functional properties. Zinc-finger CCHC domain-containing 12 () was reported to be the representative marker for a subtype of galanin-positive () DRG neurons. However, the characteristics and functions of neurons are largely unknown. Here, we genetically labeled neurons in -CreERT2::Ai9 mice, and verified that represented a new subpopulation of DRG neurons in both sexes. neurons centrally innervated the superficial laminae in spinal dorsal horn, and peripherally terminated as free nerve endings in the epidermis and cluster-shaped fibers in the dermis of footpads and nearby. In addition, neurons also formed circumferential endings surrounding the hair follicles in hairy skin. Functionally, calcium imaging in DRGs revealed that neurons were polymodal nociceptors and could be activated by mechanical and noxious thermal stimuli. Behavioral tests showed that selective ablation of DRG neurons reduced the sensitivity to noxious heat in mice. Together, we identified a new subpopulation of nociceptors essential for noxious heat sensation. represents a new subpopulation of DRG neurons. The characteristics and functions of neurons are largely unknown. Here we genetically labeled neurons, and showed that the fibers of DRG neurons projected to superficial lamina at spinal dorsal horn, and innervated skin as free nerve endings in the epidermis and cluster-shaped fibers in the dermis of footpads and nearby. Functionally, DRG neurons responded to noxious mechanical and heat stimuli. Ablation of DRG neurons impaired the sensation of noxious heat in mice. Therefore, we identify a new subpopulation of DRG neurons required for noxious heat sensation.
DRG 神经元分为不同类型，以介导不同模式的躯体感觉。最近，通过单细胞 RNA 测序对 DRG 神经元进行转录谱分析，为神经元分型和功能特性提供了新的见解。锌指 CCHC 结构域包含蛋白 12 () 被报道为甘丙肽阳性 () DRG 神经元的代表标记物。然而，神经元的特征和功能在很大程度上尚不清楚。在这里，我们在 -CreERT2::Ai9 小鼠中遗传标记了神经元，并验证了在两性中均代表了 DRG 神经元的一个新亚群。神经元在脊髓背角浅层有中枢性传入，在外周，其末端为表皮中的游离神经末梢和足垫及其附近真皮中的簇状纤维。此外，神经元还在有毛皮肤的毛囊周围形成环状终末。功能上，在 DRG 中的钙成像显示神经元是多模式伤害感受器，可被机械和有害热刺激激活。行为测试表明，选择性消融神经元减少了小鼠对有害热的敏感性。总之，我们鉴定了一个新的伤害感受神经元亚群，该亚群对于有害热感觉是必不可少的。代表 DRG 神经元的一个新亚群。神经元的特征和功能在很大程度上尚不清楚。在这里，我们对神经元进行了遗传标记，并显示神经元的纤维投射到脊髓背角的浅层，并且在表皮中作为游离神经末梢和足垫及其附近真皮中的簇状纤维支配皮肤。功能上，神经元对有害机械和热刺激有反应。消融神经元损害了小鼠对有害热的感觉。因此，我们鉴定了一个新的伤害感受神经元亚群，该亚群对于有害热感觉是必需的。
