Thanomsit Chutima, Saetiew Jadsada, Meemon Panomsak
Department of Fisheries, Faculty of Agriculture and Technology, Rajamangala University of Technology Isan Surin Campus, Surin, 32000, Thailand.
School of Physics, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand.
Toxicol Rep. 2022 Jan 29;9:181-190. doi: 10.1016/j.toxrep.2022.01.010. eCollection 2022.
Glyphosate contamination in fresh water is a major problem in agricultural countries. It affects many vital organs in freshwater organisms that are important in the food chain. Hence, the effects of glyphosate on living organism organs are of particular interest. However, several existing techniques for evaluating the effect of glyphosate on aquatic organisms require stained tissue. To study organ tissue with minimal processing, alternative technique is demanded. Here, we investigated the used of optical coherence tomography (OCT) as an alternative tool for evaluation of the effect of glyphosate on organ tissues of aquatic organisms, i.e., hybrid catfish ( × ). The targeted samples were organ tissues from the brain, gill, and liver of hybrid catfish after glyphosate exposion at concentration of 10 mg L for 24 h. The alteration was then verified by histology, and immunohistochemistry. The study found that all three techniques provide correlated results. We observed that OCT clearly showed damage to the brain and gill tissues of glyphosate-exposed hybrid catfish. However, the alteration in liver tissue was observable but not clear for this low concentration of exposure. The results from histology and immunohistochemistry confirmed the effect of glyphosate on brain, gill, and liver tissues of hybrid catfish. The results suggest that all three techniques could be used to examine the effects of glyphosate exposure in hybrid catfish. However, the choice of a suitable technique depends upon the purpose of the study.
淡水中的草甘膦污染是农业国家面临的一个主要问题。它会影响淡水生物体内许多对食物链很重要的重要器官。因此，草甘膦对生物体器官的影响备受关注。然而，现有的几种评估草甘膦对水生生物影响的技术需要对组织进行染色。为了以最少的处理来研究器官组织，需要替代技术。在此，我们研究了光学相干断层扫描（OCT）作为评估草甘膦对水生生物（即杂交鲶鱼（×））器官组织影响的替代工具的用途。目标样本是杂交鲶鱼在10毫克/升浓度的草甘膦中暴露24小时后，取自其脑、鳃和肝脏的器官组织。然后通过组织学和免疫组织化学来验证这种变化。研究发现这三种技术都能提供相关结果。我们观察到，OCT清楚地显示了暴露于草甘膦的杂交鲶鱼的脑和鳃组织受到了损伤。然而，对于这种低浓度暴露，肝脏组织的变化可以观察到但不太明显。组织学和免疫组织化学的结果证实了草甘膦对杂交鲶鱼脑、鳃和肝脏组织的影响。结果表明，这三种技术都可用于检测杂交鲶鱼中草甘膦暴露的影响。然而，合适技术的选择取决于研究目的。
