In vivo three-dimensional characterization of the adult zebrafish brain using a 1325 nm spectral-domain optical coherence tomography system with the 27 frame/s video rate.

In this study, a spectral-domain optical coherence tomography (SD-OCT) system was used for noninvasive imaging of the adult zebrafish brain. Based on a 1325 nm light source and two high-speed galvo mirrors, our SD-OCT system can offer a large field of view of brain morphology with high resolution (12 μm axial and 13 μm lateral) at video rate (27 frame/s). In vivo imaging of both the control and injured brain was performed using adult zebrafish model. The recovered results revealed that olfactory bulb, optic commissure, telencephalon, tectum opticum, cerebellum, medulla, preglomerular complex and posterior tuberculum could be clearly identified in the cross-sectional SD-OCT images of the adult zebrafish brain. The reconstructed results also suggested that SD-OCT can be used for diagnosis and monitoring of traumatic brain injury. In particular, we found the reconstructed volumetric SD-OCT images enable a comprehensive three-dimensional characterization of the control or injured brain in the intact zebrafish.