Agbohessi Prudencio, Olowo Laurence, Degila Bodelaire, Houedjissi Gisèle, Imorou Toko Ibrahim
Research Laboratory in Aquaculture and Aquatic Ecotoxicology (LaRAEAq), Faculty of Agronomy, University of Parakou, Parakou, Benin.
J Environ Sci Health B. 2023;58(1):21-30. doi: 10.1080/03601234.2022.2162797. Epub 2022 Dec 30.
Aquatic organisms are exposed to chemical pesticides including glyphosate (Sharp 480 SL) and atrazine (Atraforce), two phytocidal molecules used for agriculture purposes in Benin. In this study, we assessed the acute toxicity of these two herbicides with emphasis on their histopathological effects on the liver of catfish One hundred and eighty juveniles of (mean length 7.26 ± 0.59 cm and mean weight 5.21 ± 3.22 g) were exposed over 96 h to increasing concentrations of each phytocide. The values of 96 h-LC were 6.175 × 10 and 3.165 ppm, respectively for Sharp 480 SL and Atraforce. This indicates that Sharp 480 SL was nontoxic, while Atraforce displayed a moderate toxicity to juveniles. During the tests, the behavioral responses (hyperexcitation, lethargy, loss of balance, discoloration of skin, etc.) that usually precede death were observed in exposed fishes, confirming the neurotoxicity of these phytocides. Histological alterations observed in liver of contaminated fishes were regressive changes, such as necrosis, hepatocyte vacuolation, nuclear degeneration, hepatocytes degeneration, sinusoids dilatation, etc. These results indicate that exposure to these herbicides had destructive effects on the liver of .
水生生物会接触到化学农药，包括草甘膦（锐超麦480 SL）和莠去津（阿特拉津），这两种用于贝宁农业的杀植物分子。在本研究中，我们评估了这两种除草剂的急性毒性，并重点研究了它们对鲶鱼肝脏的组织病理学影响。180尾幼年鲶鱼（平均体长7.26±0.59厘米，平均体重5.21±3.22克）在96小时内暴露于每种除草剂浓度不断增加的环境中。锐超麦480 SL和阿特拉津的9６小时半数致死浓度分别为6.175×10和3.165 ppm。这表明锐超麦480 SL无毒，而阿特拉津对幼年鲶鱼显示出中等毒性。在测试过程中，在暴露的鱼类中观察到了通常在死亡前出现的行为反应（过度兴奋、嗜睡丧失平衡、皮肤变色等），证实了这些除草剂的神经毒性。在受污染鱼类的肝脏中观察到的组织学改变是退行性变化，如坏死、肝细胞空泡化、核变性、肝细胞变性、血窦扩张等。这些结果表明，接触这些除草剂对鲶鱼的肝脏有破坏作用。
