Roberts T M, Pavalko F M, Ward S
J Cell Biol. 1986 May;102(5):1787-96. doi: 10.1083/jcb.102.5.1787.
During the development of pseudopodial spermatozoa of the nematode, Caenorhabditis elegans, protein synthesis stops before differentiation is completed. Colloidal gold conjugates of monoclonal antibody SP56, which binds to the surface of spermatozoa, and TR20, which recognizes the major sperm cytoplasmic protein (MSP), were used to label thin sections of testes embedded in Lowicryl K4M in order to follow polypeptides from their synthesis early in spermatogenesis to their segregation to specific compartments of the mature cell. Both antigens are synthesized in primary spermatocytes and are assembled into a unique double organelle, the fibrous body-membranous organelle (FB-MO) complex. However, the antigens are localized in different regions of this FB-MO complex. As described in detail, the assembly of proteins into the FB-MO complex allows both membrane and cytoplasmic components to be concentrated in the spermatids after meiosis. Then, the stepwise disassembly of this transient structure ensures delivery of each component to its final destination in the mature spermatozoan: MSP filaments in the fibrous body depolymerize, releasing MSP into the cytoplasm and the membranous organelles fuse with the plasma membrane, delivering SP56 antigen to the surface.
在线虫秀丽隐杆线虫伪足精子发育过程中，蛋白质合成在分化完成前就停止了。与精子表面结合的单克隆抗体SP56以及识别主要精子细胞质蛋白（MSP）的TR20的胶体金缀合物，被用于标记包埋在低温包埋剂K4M中的睾丸薄片，以便追踪多肽从精子发生早期的合成到它们在成熟细胞特定区室中的分离过程。两种抗原均在初级精母细胞中合成，并组装成一种独特的双细胞器，即纤维体-膜细胞器（FB-MO）复合体。然而，抗原定位于该FB-MO复合体的不同区域。如详细描述的那样，蛋白质组装到FB-MO复合体中使得膜成分和细胞质成分在减数分裂后的精子细胞中得以浓缩。然后，这种瞬时结构的逐步解体确保了每个成分被递送到成熟精子中的最终目的地：纤维体中的MSP细丝解聚，将MSP释放到细胞质中，膜细胞器与质膜融合，将SP56抗原递送到表面。
