ART 初治的 HIV 感染的中国男男性行为者中 T 细胞亚群的加速老化：一项病例对照研究。

Accelerated Aging of T-Cell Subsets Among ART-Naïve HIV-Infected Chinese Men Who have Sex with Men: A Case-Control Study.

机构信息

Clinical and Research Center of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China.

Clinical Laboratory of Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China.

出版信息

Curr HIV Res. 2022 Aug 12;20(2):129-136. doi: 10.2174/1570162X20666220216103504.

DOI:10.2174/1570162X20666220216103504

PMID:35170409

Abstract

BACKGROUND

Evidence of lymphopoiesis, exhaustion, and premature aging in Chinese patients with human immunodeficiency virus (HIV) is very limited.

OBJECTIVE

To assess biological aging and immune senescence in Chinese healthy controls (HC) and ART-naïve HIV-infected men who have sex with men (MSM).

METHODS

This case-control study was conducted in Beijing Ditan Hospital from March 2018 to June 2019. The percentages of naïve (TN), central memory (TCM), effector memory (TEM), and terminally differentiated memory (TemRA) subsets of CD4 and CD8 T cells were studied, along with markers of senescence (CD28-CD57+) and activation (HLA-DR+). Telomere length of naïve (CD45RA+) and memory (CD45RO+) CD8 T cells were quantified by real-time PCR.

RESULTS

A total of 26 HIV-infected and 20 age-matched HC MSM were included. Compared to the HC group, the CD4/CD8 ratio of the HIV-infected group was significantly reduced (0.30 vs. 1.70, P<0.001); significant differences emerged among all CD8 but not CD4 T cell subsets (all P<0.05). In the HIV-infected group, the percentages of senescent cells (CD28-CD57+) in TN, TCM, TEM, and TemRA subsets of CD8 T cells were higher (all P<0.05); while a significant difference was only found in naïve CD4 T cells (P<0.05). HLA-DR expression was increased significantly in all CD4 and CD8 T cell subsets. Both naïve (CD45RA+) and memory (CD45RO+) CD8 T cells in this population had significantly shorter telomere lengths (P<0.01) compared to the HC group.

CONCLUSION

HIV-infected MSM exhibit signs of accelerated immune senescence and biological aging, which particularly affects the CD8 T-cell subsets.

摘要

背景

在中国艾滋病毒（HIV）感染者中，关于淋系细胞增生、衰竭和早衰的证据非常有限。

目的

评估中国健康对照（HC）和未经抗逆转录病毒治疗（ART）的 HIV 感染男男性行为者（MSM）中的生物衰老和免疫衰老。

方法

这项病例对照研究于 2018 年 3 月至 2019 年 6 月在北京地坛医院进行。研究了 CD4 和 CD8 T 细胞的幼稚（TN）、中央记忆（TCM）、效应记忆（TEM）和终末分化记忆（TemRA）亚群的比例，以及衰老（CD28-CD57+）和激活（HLA-DR+）标志物。通过实时 PCR 定量测定幼稚（CD45RA+）和记忆（CD45RO+）CD8 T 细胞的端粒长度。

结果

共纳入 26 例 HIV 感染者和 20 名年龄匹配的 HC MSM。与 HC 组相比，HIV 感染者组的 CD4/CD8 比值显著降低（0.30 比 1.70，P<0.001）；所有 CD8 T 细胞亚群（均 P<0.05）之间均有显著差异，而 CD4 T 细胞亚群无显著差异。在 HIV 感染者中，CD8 T 细胞的幼稚（TN）、中央记忆（TCM）、效应记忆（TEM）和终末分化记忆（TemRA）亚群中衰老细胞（CD28-CD57+）的比例较高（均 P<0.05）；而幼稚 CD4 T 细胞仅存在显著差异（P<0.05）。所有 CD4 和 CD8 T 细胞亚群的 HLA-DR 表达均显著增加。与 HC 组相比，该人群中的幼稚（CD45RA+）和记忆（CD45RO+）CD8 T 细胞的端粒长度明显缩短（均 P<0.01）。