COMSATS University Islamabad, Department of Pharmacy, Abbottabad, Pakistan.
Hamdard University, Hamdard Institute of Pharmaceutical Sciences, Karachi, Islamabad, Pakistan.
Braz J Biol. 2022 Feb 11;82:e257990. doi: 10.1590/1519-6984.257990. eCollection 2022.
Bauhinia variegata plant is a very popular and traditionally potent ethnomedicine. Therefore, it is need of hour to study ameliorative characteristics of B. variegata for novel secondary metabolites. The current study was designed to explore antiproliferative potential of B. variegata due to scant reports on this aspect. Extracts of various parts (flowers, leaves, bark, stem, and roots) were prepared by successive maceration using organic solvents in increasing order of polarity (n-hexane, ethyl acetate, methanol, and water). The determination of polyphenolic contents was done by using colorimetric methods while antioxidant potential was measured using reducing power assay. Brine shrimp lethality assay was performed for determining preliminary cytotoxicity and antiproliferative activity against breast cancer MCF-7 cell line using MTT protocols. Moreover, antimicrobial activities were detected by using disc diffusion assay. The alpha-amylase assay was performed to monitor the antidiabetic potential of the plant. In case of phytochemical analysis methanolic extract of leaves and bark showed highest phenolic and flavonoids contents. n-Hexane and ethyl acetate extracts of stem and roots exhibited more than 90% mortality with LD50 ranges between 1-25 µg/mL when studied by brine shrimp lethality assay. n-Hexane and ethyl acetate extracts of roots and stem also showed antiproliferative activity against human breast cancer MCF-7 cell line with IC50 values ranges between 12.10-14.20 µg/mL. Most of the extracts displayed moderately high antibacterial and antifungal activities. The n-hexane extract of roots showed antidiabetic activity with 60.80 ± 0.20% inhibition of alpha-amylase. In sum, these preliminary results will be useful for further compound isolation from selected plant parts for the discovery of antibacterial, antidiabetic, and anticancer lead candidates.
美丽异木棉植物是一种非常受欢迎且具有传统功效的民族药物。因此，迫切需要研究美丽异木棉的改良特性，以寻找新的次生代谢物。本研究旨在探索美丽异木棉的抗增殖潜力，因为这方面的报道很少。采用连续浸渍法，用有机溶剂从低极性到高极性依次提取（正己烷、乙酸乙酯、甲醇和水）各部位（花、叶、树皮、茎和根）的提取物。采用比色法测定多酚含量，采用还原力测定法测定抗氧化潜力。采用盐水虾致死试验测定初步细胞毒性和对乳腺癌 MCF-7 细胞系的抗增殖活性，采用 MTT 法。此外，还通过圆盘扩散试验检测了抗菌活性。采用α-淀粉酶测定法监测植物的降血糖潜力。在植物化学分析中，叶和树皮的甲醇提取物显示出最高的酚类和类黄酮含量。通过盐水虾致死试验研究发现，茎和根的正己烷和乙酸乙酯提取物的死亡率超过 90%，LD50 范围在 1-25μg/mL 之间。茎和根的正己烷和乙酸乙酯提取物对人乳腺癌 MCF-7 细胞系也表现出抗增殖活性，IC50 值范围在 12.10-14.20μg/mL 之间。大多数提取物表现出中等强度的抗菌和抗真菌活性。根的正己烷提取物具有降血糖活性，对α-淀粉酶的抑制率为 60.80±0.20%。总之，这些初步结果将有助于从选定的植物部分进一步分离化合物，以发现抗菌、抗糖尿病和抗癌的先导候选物。
