无水磷酸钙晶体可稳定 DNA 以供干燥储存。
Anhydrous calcium phosphate crystals stabilize DNA for dry storage.
机构信息
Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zurich, Vladimir-Prelog-Weg 1, 8093 Zürich, Switzerland.
Laboratory for Catalysis and Sustainable Chemistry, Paul Scherrer Institute, Forschungsstrasse 111, 5232 Villigen, Switzerland.
出版信息
Chem Commun (Camb). 2022 Mar 3;58(19):3174-3177. doi: 10.1039/d2cc00414c.
The resilience of ancient DNA (aDNA) in bone gives rise to the preservation of synthetic DNA with bioinorganic materials such as calcium phosphate (CaP). Accelerated aging experiments at elevated temperature and humidity displayed a positive effect of co-precipitated, crystalline dicalcium phosphate on the stability of synthetic DNA in contrast to amorphous CaP. Quantitative PXRD in combination with SEM and EDX measurements revealed distinct CaP phase transformations of calcium phosphate dihydrate (brushite) to anhydrous dicalcium phosphate (monetite) influencing DNA stability.
骨骼中古老 DNA（aDNA）的弹性使得与生物无机材料（如磷酸钙（CaP））结合的合成 DNA 得以保存。在高温高湿度下进行的加速老化实验显示，与非晶态 CaP 相比，共沉淀的结晶二水磷酸钙（brushite）对合成 DNA 的稳定性有积极影响。定量 PXRD 结合 SEM 和 EDX 测量揭示了磷酸氢钙二水合物（brushite）向无水二水磷酸钙（monetite）的明显 CaP 相变，影响 DNA 的稳定性。
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