Department of General Surgery, People's Hospital of Baoan District, No.118 Longjing Second Road, Baoan District, Shenzhen City, 518101, People's Republic of China.
Bone and Joint Surgery, Shenzhen Baoan Shiyan People's Hospital, Shenzhen City, 518101, People's Republic of China.
In Vitro Cell Dev Biol Anim. 2022 Feb;58(2):109-115. doi: 10.1007/s11626-021-00642-x. Epub 2022 Feb 16.
LINC 01436 (lncRNA) promotes lung and gastric cancers. However, it is unclear whether it participates in colorectal cancer (CRC) progression. Therefore, the study was carried out to analyze the role of LINC 01436 in CRC. LINC 01436 expressions in CRC tissues were analyzed by RT-qPCR, and its prognostic value was investigated in a follow-up study. Correlation between LINC 01436 and mature miR-466 or miR-466 precursor was analyzed by linear regression. Mature miR-466 and miR-466 precursor levels in CRC cells with LINC 01436 overexpression were studied using RT-qPCR. CRC cell proliferation was evaluated using CCK-8 assay. LINC 01436 was upregulated in CRC and predicted poor survival. LINC 01436 was inversely correlated with mature miR-466, but not miR-466 precursor. LINC 01436 was predicted to bind with miR-466 precursor. Their interaction was further verified by dual-luciferase activity assay. In CRC cells, LINC 01436 overexpression downregulated mature miR-466 but not miR-466 precursor. Cell proliferation analysis showed that LINC 01436 overexpression rescued cell proliferation reduced by miR-466. LINC 01436 is overexpressed in CRC and promotes cancer cell proliferation by suppressing miR-466 maturation.
LINC 01436（lncRNA）促进肺癌和胃癌。然而，它是否参与结直肠癌（CRC）的进展尚不清楚。因此，本研究旨在分析 LINC 01436 在 CRC 中的作用。通过 RT-qPCR 分析 CRC 组织中的 LINC 01436 表达，并在随访研究中研究其预后价值。通过线性回归分析 LINC 01436 与成熟 miR-466 或 miR-466 前体之间的相关性。使用 RT-qPCR 研究过表达 LINC 01436 的 CRC 细胞中成熟 miR-466 和 miR-466 前体的水平。使用 CCK-8 测定法评估 CRC 细胞增殖。LINC 01436 在 CRC 中上调并预测预后不良。LINC 01436 与成熟 miR-466 呈负相关，但与 miR-466 前体无关。LINC 01436 被预测与 miR-466 前体结合。通过双荧光素酶活性测定进一步验证了它们的相互作用。在 CRC 细胞中，LINC 01436 的过表达下调了成熟的 miR-466，但未下调 miR-466 前体。细胞增殖分析表明，LINC 01436 的过表达挽救了 miR-466 降低的细胞增殖。LINC 01436 在 CRC 中过表达，并通过抑制 miR-466 的成熟来促进癌细胞增殖。
