Laboratory of Nutritional Brain Science, Department of Food Science and Nutrition, Mukogawa Women's University, 6-46 Ikebiraki, Nishinomiya, Hyogo, 663-8558, Japan.
BMC Res Notes. 2022 Feb 16;15(1):60. doi: 10.1186/s13104-022-05954-z.
More than half of patients with depression display eating disorders, such as bulimia nervosa and anorexia nervosa. Feeding centers are located in the hypothalamus, and hypothalamic adult neurogenesis has an important role in feeding and energy balance. Antidepressants, which can regulate adult neurogenesis in the hippocampus, olfactory bulb, and neocortex, are used for eating disorders, but it is unclear whether antidepressants change hypothalamic adult neurogenesis. In this study, we used immunohistological analysis to assess effects of the antidepressant fluoxetine (FLX) on hypothalamic adult neurogenesis of adult mice.
Expressions of the proliferating cell marker, Ki67, and the neural stem cell marker, nestin, were significantly decreased in the hypothalamus by FLX. As regard to postmitotic cells, the number of the neural marker, NeuN, positive cells was significantly upregulated by FLX, but that of the astrocytic marker, S100B, positive cells was significantly reduced by FLX. The number of the oligodendrocyte marker, Olig2, positive cells was not changed by FLX. Interestingly, FLX treatment did not affect the total number of newly generated cells in the hypothalamus, comparing that in controls. These results suggest that FLX treatment influence hypothalamic adult neurogenesis and shift the balance between the numbers of neurons and astrocytes under studied conditions.
超过一半的抑郁症患者表现出饮食失调，如神经性贪食症和神经性厌食症。摄食中枢位于下丘脑，而下丘脑成体神经发生在摄食和能量平衡中起着重要作用。抗抑郁药可以调节海马体、嗅球和新皮质中的成体神经发生，用于治疗饮食失调，但目前尚不清楚抗抑郁药是否会改变下丘脑的成体神经发生。在这项研究中，我们使用免疫组织化学分析来评估抗抑郁药氟西汀（FLX）对成年小鼠下丘脑成体神经发生的影响。
FLX 显著降低了下丘脑内增殖细胞标志物 Ki67 和神经干细胞标志物巢蛋白的表达。就有丝分裂后细胞而言，FLX 显著上调了神经标志物 NeuN 阳性细胞的数量，但下调了星形胶质细胞标志物 S100B 阳性细胞的数量。FLX 对少突胶质细胞标志物 Olig2 阳性细胞的数量没有影响。有趣的是，FLX 处理并没有影响与对照组相比新生成的细胞在下丘脑的总数。这些结果表明，FLX 处理影响下丘脑成体神经发生，并在研究条件下改变神经元和星形胶质细胞数量之间的平衡。
