微小RNA-1179通过抑制NUAK2来抑制肝细胞癌的细胞增殖、迁移和侵袭。
MiR-1179 represses cell proliferation, migration and invasion of hepatocellular carcinoma through suppression of NUAK2.
作者信息
Wang Dejun, Song Xue, Zhang Nan, Guo Yesong
机构信息
Department of Radiotherapy, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University Nanjing, Jiangsu Province, China.
出版信息
Am J Transl Res. 2022 Jan 15;14(1):223-239. eCollection 2022.
OBJECTIVE
To investigate the function and mechanism of miR-1179 in the tumorigenesis of hepatocellular carcinoma (HCC).
METHODS
The levels of miR-1179 and NUAK2 in clinical tissues or cell lines were examined using quantitative Real-time PCR (qRT-PCR). Cell Counting Kit-8 (CCK-8), EdU assay, colony formation, wound healing, Transwell assays and flow cytometry assays were conducted to examine the impact of miR-1179 on HCC cells. The protein expression of NUAK2 was detected using Western blotting assay. Bioinformatics analysis and luciferase reporter assays were conducted to reveal the association of miR-1179 with NUAK2.
RESULTS
MiR-1179 expression was significantly downregulated in HCC specimens and cell lines compared to normal samples and cells. The miR-1179 overexpression inhibited HCC cell migration, invasion and proliferation through targeting NUAK2. Overexpression of NUAK2 can reverse the effect of miR-1179 on hepatocellular carcinoma cells.
CONCLUSION
miR-1179 suppresses HCC developmen through targeting NUAK2. Which can be used as a potent HCC diagnostic marker.
目的
研究miR-1179在肝细胞癌（HCC）肿瘤发生中的作用及机制。
方法
采用定量实时荧光定量PCR（qRT-PCR）检测临床组织或细胞系中miR-1179和NUAK2的水平。进行细胞计数试剂盒-8（CCK-8）、EdU检测、集落形成、伤口愈合、Transwell检测和流式细胞术检测，以研究miR-1179对肝癌细胞的影响。采用蛋白质免疫印迹法检测NUAK2的蛋白表达。通过生物信息学分析和荧光素酶报告基因检测揭示miR-1179与NUAK2的关系。
结果
与正常样本和细胞相比，HCC标本和细胞系中miR-1179表达明显下调。miR-1179过表达通过靶向NUAK2抑制HCC细胞迁移、侵袭和增殖。NUAK2过表达可逆转miR-1179对肝癌细胞的作用。
结论
miR-1179通过靶向NUAK2抑制HCC发展。其可作为一种有效的HCC诊断标志物。
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