Nimaev V V, Pivkina A V, Shurlygina A V, Rachkovskaya L N, Smagin A A, Yastrebova E S, Rachkovskii E E, Korolev M A, Mal'tsev V P, Letyagin A Yu
Research Institute of Clinical and Experimental Lymphology - Affiliated Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Bull Exp Biol Med. 2022 Feb;172(4):478-482. doi: 10.1007/s10517-022-05417-2. Epub 2022 Feb 17.
A comparative study of the effect of a sorbent with nanotubes (AlO@ WCNT-PDMS) and a carbon-mineral sorbent (AlO@C) on the parameters of human erythrocytes was carried out. Using scanning flow cytometry, the morphological and functional parameters of venous blood erythrocytes as well as drainage blood after its perfusion through columns with sorbents were determined. The compared samples AlO@SWCNT-PDMS and AlO@C are similar by their effect on the morphological and functional parameters of erythrocytes. The maximum membrane extensibility increased to a greatest extent after contact with AlO@C, the amount of hemoglobin in erythrocytes decreased to the greatest extent after perfusion through a column with AlO@SWCNT-PDMS sorbent. The scanning flow cytometry is promising for assessing the effect on erythrocytes of new sorption materials intended for blood detoxification. Changes in the parameters of erythrocytes of blood collected in a sterile drainage system for subsequent reinfusion were revealed.
开展了一项关于含纳米管吸附剂（AlO@WCNT-PDMS）和碳-矿物吸附剂（AlO@C）对人体红细胞参数影响的对比研究。使用扫描流式细胞术，测定了静脉血红细胞以及通过装有吸附剂的柱体灌注后的引流血的形态和功能参数。所比较的样品AlO@SWCNT-PDMS和AlO@C对红细胞形态和功能参数的影响相似。与AlO@C接触后，最大膜伸展性增加幅度最大；通过装有AlO@SWCNT-PDMS吸附剂的柱体灌注后，红细胞内血红蛋白含量下降幅度最大。扫描流式细胞术在评估用于血液解毒的新型吸附材料对红细胞的影响方面具有前景。还揭示了在无菌引流系统中采集以供后续回输的血液红细胞参数的变化。
