Tang M S, Hrncir J, Mitchell D, Ross J, Clarkson J
Mutat Res. 1986 Jun;161(1):9-17. doi: 10.1016/0027-5107(86)90095-3.
In order to calculate the relative cytotoxicity and mutagenicity of (5-6) cyclobutane pyrimidine dimers and (6-4) photoproducts, we have measured survival and mutation induction in UV-irradiated excision-deficient E. coli uvrA cells, with or without complete photoreactivation of the (5-6) dimers. Radioimmunoassays with specificity for (5-6) dimers or (6-4) photoproducts have shown that maximum photoreactivation eliminates all of the (5-6) dimers produced up to 10 Jm-2 254-nm light, while it has no effect on (6-4) photoproducts. These results were confirmed by measuring the frequency of T4 endonuclease V-sensitive sites. Based on the best fit equations for survival and mutation induction, we have found that the calculated cytotoxicity of (6-4) photoproducts is similar to that of (5-6) dimers; however, the former is much more mutagenic than the latter.
为了计算（5-6）环丁烷嘧啶二聚体和（6-4）光产物的相对细胞毒性和致突变性，我们测定了紫外线照射的切除缺陷型大肠杆菌uvrA细胞中的存活率和突变诱导情况，（5-6）二聚体有无完全光复活。对（5-6）二聚体或（6-4）光产物具有特异性的放射免疫分析表明，最大程度的光复活消除了高达10 Jm-2 254纳米光产生的所有（5-6）二聚体，而对（6-4）光产物没有影响。通过测量T4内切核酸酶V敏感位点的频率证实了这些结果。基于存活率和突变诱导的最佳拟合方程，我们发现计算得出的（6-4）光产物的细胞毒性与（5-6）二聚体相似；然而，前者的致突变性比后者大得多。
