Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, NY 10065, USA. Electronic address: https://twitter.com/AVBroeck.
Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, NY 10065, USA.
Curr Opin Struct Biol. 2022 Apr;73:102331. doi: 10.1016/j.sbi.2022.102331. Epub 2022 Feb 14.
The biogenesis of the eukaryotic ribosome is a tightly regulated and energetically demanding process involving more than 200 ribosome assembly factors. These factors work in concert to ensure accurate assembly and maturation of both ribosomal subunits. Cryo-electron microscopy (cryo-EM) structures of numerous eukaryotic ribosome assembly intermediates have provided a wealth of structural insights highlighting the molecular interplay of a cast of assembly factors. In this review, we focus on recently determined structures of maturing small subunit (SSU) processomes, giant precursors of the small ribosomal subunit. Based on these structures and complementary biochemical and genetic studies, we discuss an emerging mechanism involving exosome-mediated SSU processome maturation and disassembly.
真核核糖体的生物发生是一个受到严格调控和能量需求高的过程，涉及 200 多种核糖体组装因子。这些因子协同工作，以确保核糖体亚基的准确组装和成熟。大量真核核糖体组装中间产物的冷冻电子显微镜（cryo-EM）结构提供了丰富的结构见解，突出了组装因子的分子相互作用。在这篇综述中，我们重点介绍了最近确定的成熟小亚基（SSU）加工体的结构，这是小核糖体亚基的巨大前体。基于这些结构以及互补的生化和遗传研究，我们讨论了一种新出现的机制，涉及外切体介导的 SSU 加工体成熟和解体。
