Sato José Paulo H, Daniel Amanda G S, Leal Carlos A G, Barcellos David E S N, Guedes Roberto M C
Department of Clinical and Veterinary Surgery, Veterinary School, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Department of Preventive Veterinary Medicine, Veterinary School, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Vet Microbiol. 2022 Mar;266:109369. doi: 10.1016/j.vetmic.2022.109369. Epub 2022 Feb 11.
The aim of this study was to evaluate genetic diversity, distribution, evolution and population structure of Brazilian Brachyspira hyodysenteriae strains isolated from swine. Multilocus Sequence Typing (MLST) analysis using seven housekeeping genes was applied to 46 isolates obtained from outbreaks of swine dysentery that occurred between 2011 and 2015 in the states of Minas Gerais, São Paulo, Mato Grosso, Rio Grande do Sul and Santa Catarina. Historical isolates from Rio Grande do Sul obtained in 1998 were also included in the study. An independent international profile of the global sequences deposited in the B. hyodysenteriae database was used for comparisons with the Brazilian strains. All isolates from 2011 to 2015 were classified into nine sequence type (STs) and divided into four clonal complexes. These findings indicated genetic relationships among the B. hyodysenteriae from different Brazilian states, among historical strains isolated in 1998 and from recent outbreaks, and relatedness with global isolates. Seven STs were unique and, to date, only reported in Brazil.
本研究旨在评估从猪身上分离出的巴西猪痢疾短螺旋体菌株的遗传多样性、分布、进化和种群结构。运用多位点序列分型（MLST）分析法，对2011年至2015年期间在米纳斯吉拉斯州、圣保罗州、马托格罗索州、南里奥格兰德州和圣卡塔琳娜州发生的猪痢疾疫情中分离出的46株菌株，使用7个管家基因进行分析。1998年从南里奥格兰德州获得的历史菌株也纳入了本研究。利用猪痢疾短螺旋体数据库中保存的全球序列的独立国际图谱，与巴西菌株进行比较。2011年至2015年的所有分离株被分为9个序列型（STs），并分为4个克隆复合体。这些结果表明了来自巴西不同州的猪痢疾短螺旋体之间、1998年分离的历史菌株与近期疫情菌株之间的遗传关系，以及与全球分离株的相关性。7个STs是独特的，迄今为止仅在巴西报道过。
