Williams Katherine, de Mattos-Shipley Kate M J, Willis Christine L, Bailey Andrew M
School of Biological Sciences, Life Sciences Building, University of Bristol, 24 Tyndall Ave, Bristol, BS8 1TQ, UK.
School of Chemistry, University of Bristol, Cantock's Close, Bristol, BS8 1TS, UK.
Fungal Biol Biotechnol. 2022 Feb 17;9(1):2. doi: 10.1186/s40694-022-00132-z.
Maleidrides are a family of structurally related fungal natural products, many of which possess diverse, potent bioactivities. Previous identification of several maleidride biosynthetic gene clusters, and subsequent experimental work, has determined the 'core' set of genes required to construct the characteristic medium-sized alicyclic ring with maleic anhydride moieties. Through genome mining, this work has used these core genes to discover ten entirely novel putative maleidride biosynthetic gene clusters, amongst both publicly available genomes, and encoded within the genome of the previously un-sequenced epiheveadride producer Wicklowia aquatica CBS 125634. We have undertaken phylogenetic analyses and comparative bioinformatics on all known and putative maleidride biosynthetic gene clusters to gain further insights regarding these unique biosynthetic pathways.
马来酰亚胺类化合物是一类结构相关的真菌天然产物，其中许多具有多样的强效生物活性。先前对几个马来酰亚胺生物合成基因簇的鉴定以及随后的实验工作，已经确定了构建具有马来酸酐部分的特征性中等大小脂环族环所需的“核心”基因集。通过基因组挖掘，这项工作利用这些核心基因在公开可用的基因组以及先前未测序的表环氧马来酰亚胺产生菌水生威克洛菌CBS 125634的基因组中发现了十个全新的假定马来酰亚胺生物合成基因簇。我们对所有已知和假定的马来酰亚胺生物合成基因簇进行了系统发育分析和比较生物信息学研究，以进一步了解这些独特的生物合成途径。
