State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong 266237, China.
Nat Prod Rep. 2021 Jun 23;38(6):1072-1099. doi: 10.1039/d1np00004g.
Covering: 2015 to the end of 2020 Fungal-derived polyketides, non-ribosomal peptides, terpenoids and their hybrids contribute significantly to the chemical space of total natural products. Cytochrome P450 enzymes play essential roles in fungal natural product biosynthesis with their broad substrate scope, great catalytic versatility and high frequency of involvement. Due to the membrane-bound nature, the functional and mechanistic understandings for fungal P450s have been limited for quite a long time. However, recent technical advances, such as the efficient and precise genome editing techniques and the development of several filamentous fungal strains as heterologous P450 expression hosts, have led to remarkable achievements in fungal P450 studies. Here, we provide a comprehensive review to cover the most recent progresses from 2015 to 2020 on catalytic functions and mechanisms, research methodologies and remaining challenges in the fast-growing field of fungal natural product biosynthetic P450s.
2015 年至 2020 年底真菌来源的聚酮化合物、非核糖体肽、萜类化合物及其杂合化合物极大地丰富了天然产物的化学空间。细胞色素 P450 酶在真菌天然产物生物合成中发挥着重要作用，其具有广泛的底物范围、巨大的催化多样性和高频参与。由于其膜结合的性质，真菌 P450 的功能和机制理解在相当长的一段时间内受到限制。然而，最近的技术进步，如高效、精确的基因组编辑技术以及几种丝状真菌菌株作为异源 P450 表达宿主的发展，使得真菌 P450 研究取得了显著的成就。在这里，我们提供了一个全面的综述，涵盖了 2015 年至 2020 年在催化功能和机制、研究方法以及真菌天然产物生物合成 P450 领域快速发展中仍然存在的挑战方面的最新进展。
