Jamilus Research Centre for Sustainable Construction (JRC), Faculty of Civil and Environmental Engineering, Universiti Tun Hussein Onn Malaysia, 86400, Parit Raja, Johor, Malaysia.
Division of Bioprocess, School of Industrial Technology, Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia.
Sci Rep. 2022 Feb 17;12(1):2682. doi: 10.1038/s41598-022-05659-0.
The self-healing of bio-concrete cracks and pores have been utilised worldwide to improve the properties of bio-concrete using different types of bacteria. Meanwhile, no published research was conducted to heal bio-foamed concrete bricks (B-FCB) pores using Bacillus tequilensis. Previous studies focused on the concentration of bacteria and neglect other factors that could affect the healing process. This research aimed to optimise the healing ratio of B-FCB pores using four factors: B. tequilensis concentration, concrete density, temperature and CO concentration. Initial water absorption (IWA) and water absorption (WA) were used as responses in statistical methods, namely, factorial and response surface methodology (RSM). B. tequilensis species was isolated from cement kiln dust, produced in a powder form, then subjected to simulate test using a special medium consisting of foamed concrete materials to check the survival ability in B-FCB. SEM, EDX, and XRD were used to investigate the healing process of B-FCB pores. The results revealed that the decrement ratios of IWA and WA of B-FCB were 52.8% and 29.1% compared to FCB, respectively. SEM results reflect the healing that occurred in B-FCB pores, mostly healed via precipitation of CaCO as demonstrated on the XRD results.
生物混凝土裂缝和孔隙的自愈合已在全球范围内得到应用,以改善生物混凝土的性能,使用不同类型的细菌。同时,尚未有研究使用解硫胺素芽孢杆菌来修复生物泡沫混凝土砖(B-FCB)的孔隙。先前的研究集中在细菌的浓度上,而忽略了可能影响愈合过程的其他因素。本研究旨在使用 4 个因素来优化 B-FCB 孔隙的愈合率:解硫胺素芽孢杆菌浓度、混凝土密度、温度和 CO2 浓度。初始吸水率(IWA)和吸水率(WA)被用作统计方法的响应,即析因和响应面法(RSM)。解硫胺素芽孢杆菌是从水泥窑灰中分离出来的,呈粉末状,然后用特殊的培养基进行模拟试验,该培养基由泡沫混凝土材料组成,以检查其在 B-FCB 中的生存能力。使用 SEM、EDX 和 XRD 研究了 B-FCB 孔隙的愈合过程。结果表明,与普通泡沫混凝土(FCB)相比,B-FCB 的 IWA 和 WA 的递减率分别为 52.8%和 29.1%。SEM 结果反映了 B-FCB 孔隙中的愈合情况,主要通过 CaCO3 的沉淀来实现,XRD 结果也证明了这一点。