Department of Otolaryngology-Head and Neck Surgery and Shanghai Key Laboratory of Sleep Disordered Breathing and Otolaryngology Institute of Shanghai Jiao Tong University, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
Department of Otorhinolaryngology and National Health Commission (NHC) Key Laboratory of Otorhinolaryngology, Shandong University Affiliated Qilu Hospital, Jinan, China.
Front Endocrinol (Lausanne). 2022 Feb 1;13:820939. doi: 10.3389/fendo.2022.820939. eCollection 2022.
Chronic intermittent hypoxia (CIH) and chronic sleep fragmentation (CSF) are two cardinal pathological features of obstructive sleep apnea (OSA). Dietary obesity is a crucial risk intermediator for OSA and metabolic disorders. Gut microbiota affect hepatic and adipose tissue morphology under conditions of CIH or CSF through downstream metabolites. However, the exact relationship is unclear. Herein, chow and high-fat diet (HFD)-fed mice were subjected to CIH or CSF for 10 weeks each and compared to normoxia (NM) or normal sleep (NS) controls. 16S rRNA amplicon sequencing, untargeted liquid chromatography-tandem mass spectrometry, and histological assessment of liver and adipose tissues were used to investigate the correlations between the microbiome, metabolome, and lipid metabolism under CIH or CSF condition. Our results demonstrated that CIH and CSF regulate the abundance of intestinal microbes (such as , spp., spp., spp.) and functional metabolites, such as tryptophan, free fatty acids, branched amino acids, and bile acids, which influence adipose tissue and hepatic lipid metabolism, and the level of lipid deposition in tissues and peripheral blood. In conclusion, CIH and CSF adversely affect fecal microbiota composition and function, and host metabolism; these findings provide new insight into the independent and synergistic effects of CIH, CSF, and HFD on lipid disorders.
慢性间歇性低氧（CIH）和慢性睡眠碎片化（CSF）是阻塞性睡眠呼吸暂停（OSA）的两个主要病理特征。饮食肥胖是 OSA 和代谢紊乱的一个重要风险中介。在 CIH 或 CSF 条件下，肠道微生物群通过下游代谢物影响肝脏和脂肪组织的形态。然而，确切的关系尚不清楚。在此，我们将 Chow 和高脂肪饮食（HFD）喂养的小鼠分别进行 10 周的 CIH 或 CSF 处理，并与常氧（NM）或正常睡眠（NS）对照组进行比较。使用 16S rRNA 扩增子测序、非靶向液相色谱-串联质谱和肝、脂肪组织的组织学评估来研究微生物组、代谢组和脂质代谢在 CIH 或 CSF 条件下的相关性。我们的研究结果表明，CIH 和 CSF 调节肠道微生物（如 、 、 、 等）和功能性代谢物（如色氨酸、游离脂肪酸、支链氨基酸和胆汁酸）的丰度，这些代谢物影响脂肪组织和肝脏的脂质代谢，以及组织和外周血中脂质沉积的水平。总之，CIH 和 CSF 对粪便微生物群组成和功能以及宿主代谢产生不利影响；这些发现为 CIH、CSF 和 HFD 对脂质紊乱的独立和协同作用提供了新的见解。
