（唇形科）叶提取物及其馏分的体外抗脂和抗血栓活性。
In Vitro Antilipidic and Antithrombotic Activities of (Lamiaceae) Leaves Extracts and Fractions.
机构信息
Department of Animal Biology, Faculty of Sciences, University of Dschang, Cameroon.
Centre for Research on Medicinal Plants and Traditional Medicine (CRPMT), Institute of Medical Research and Medicinal Plants Studies (IMPM), Yaoundé, Cameroon.
出版信息
Biomed Res Int. 2022 Feb 7;2022:4145659. doi: 10.1155/2022/4145659. eCollection 2022.
OBJECTIVE
The present study investigated the effect of the leaves extracts and fractions of on the inhibition of pancreatic lipase, cholesterol esterase, adipocytes lipid uptake, and antithrombotic activity which may be important in atherosclerosis development.
METHODS
Aqueous, ethanolic, and hydroethanolic extracts of were prepared by maceration. The hydroethanolic extract was fractionated into -hexane, ethylacetate, and butanol fractions and their inhibition of pancreatic lipase, cholesterol esterase, adipocytes lipid uptake, and antithrombotic activities measured. Liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis was carried out to determine phytochemical constituents present in the extracts.
RESULTS
The standard orlistat exhibited a higher inhibitory activity on pancreatic lipase and cholesterol esterase (16.31 g/mL and 15.75 g/mL, respectively) compared to ethyl acetate fraction (IC, 17.70 g/mL and IC, 24.8 g/mL, respectively). Among crude extract, hydroethanolic extract showed a better inhibition against pancreatic lipase (IC, 21.06 g/mL) and cholesterol esterase (IC, 25.14 g/mL) though not comparable to the effect of orlistat. The best lipid uptake inhibition was observed in the hydroethanolic extract (IC, 45.42 g/mL) followed by the ethyl acetate fraction (IC, 47.77 g/mL). A better antithrombolytic activity was exhibited by the ethyl acetate fraction at all concentrations (50-800 /mL), while hydroethanolic extract exhibited the best activity among crude extract. However, these were not comparable to the standard aspirin. The LC-HRMS analysis revealed the presence of 7--methyl luteolin 5---D-glucopyranoside, chrysoeriol 5---D-glucopyranoside, 5,7-dihydroxy-3,2',4'-trimethoxyflavone, and plectranmicin as major compounds in both hydroethanolic extract and ethyl acetate fraction.
CONCLUSION
Thus, our finding supports the traditional use of this plant, which might provide a potential source for future antiatherosclerotic drug discovery.
目的
本研究旨在探讨柳叶菜属植物叶提取物及各部位对胰脂肪酶、胆固醇脂酶、脂肪细胞摄取脂质及抗血栓形成活性的抑制作用，这些作用可能与动脉粥样硬化的发展有关。
方法
采用浸渍法制备柳叶菜属植物的水提物、醇提物和水醇提物。将水醇提物进一步分为正己烷、乙酸乙酯和正丁醇部位，并测定其对胰脂肪酶、胆固醇脂酶、脂肪细胞摄取脂质及抗血栓形成活性的抑制作用。采用液相色谱-高分辨质谱（LC-HRMS）分析鉴定提取物中的植物化学成分。
结果
标准奥利司他对胰脂肪酶和胆固醇脂酶的抑制活性（IC₅₀值分别为 16.31μg/mL 和 15.75μg/mL）明显高于乙酸乙酯部位（IC₅₀值分别为 17.70μg/mL 和 24.80μg/mL）。在粗提取物中，水醇提物对胰脂肪酶（IC₅₀值为 21.06μg/mL）和胆固醇脂酶（IC₅₀值为 25.14μg/mL）的抑制作用虽不如奥利司他，但效果更好。水醇提物对脂质摄取的抑制作用最强（IC₅₀值为 45.42μg/mL），其次是乙酸乙酯部位（IC₅₀值为 47.77μg/mL）。在所有浓度（50-800μg/mL）下，乙酸乙酯部位均表现出较好的抗血栓形成活性，而水醇提物在粗提取物中表现出最好的活性。但这些都不如标准阿司匹林。LC-HRMS 分析表明，水醇提物和乙酸乙酯部位中均含有 7--甲基木樨草素 5---D-葡萄糖苷、芫花素 5---D-葡萄糖苷、5,7-二羟基-3,2',4'-三甲氧基黄酮和刺槐素作为主要化合物。
结论
因此，我们的研究结果支持该植物的传统用途，这可能为未来抗动脉粥样硬化药物的发现提供潜在来源。
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