Maqsood Maria, Ahmed Dildar, Atique Iqra, Malik Wajeeha
Department of Chemistry, Forman Christian College (A Chartered University), Lahore, Pakistan.
Department of Chemistry, Forman Christian College (A Chartered University), Lahore, Pakistan.
Asian Pac J Trop Med. 2017 Mar;10(3):305-310. doi: 10.1016/j.apjtm.2017.03.010. Epub 2017 Mar 6.
To explore pancreatic lipase inhibitory activity under different extraction conditions in order to track the most potent extract.
The methanolic extract and its fractions in solvents of increasing polarity, ether, chloroform, ethyl acetate, n-butanol and water, were made through cold maceration. Extracts in ethanol, ethyl acetate, acetone and chloroform were similarly prepared. Aqueous extract was prepared through hot decoction method. A reported method was used to determine lipase inhibitory activity of extracts and fractions over wide ranges of concentrations.
The extracts and fractions exhibited concentration dependent activity. The IC (μg/mL) values of methanolic, ethanolic, chloroform, ethyl acetate, acetone, ethyl acetate (after washing with water) and aqueous decoction were 293.40, 266.47, 157.59, 182.12, 352.34, 257.00, and 190.00, respectively. The activity of chloroform, ethyl acetate and aqueous extracts were close to that of the drug orlistat (IC 146 μg/mL). Out of the fractions of the methanolic extract, the chloroform fraction was most active (IC 189.6 μg/mL). The order of inhibitory activity of the fractions was as follows: chloroform＞ether＞n-butanolic＞aqueous＞ethyl acetate. The GC/MS analysis of the most active chloroform faction showed the presence of hexadecanoic acid, methyl hexadecanoate, isopropyl palmitate, methyl 9,12-octadecadienate, and methyl 9,12,15-octadecatrienoate.
The study suggests that Lagenaria siceraria has potential to inhibit pancreatic lipase activity, suppressing lipid digestion and thereby diminishing entry of lipids into the body. Regular intake of aqueous decoction of the fruit may therefore be recommended for control of obesity. Fatty acids and their esters may play role as inhibitors of lipase.
探索不同提取条件下的胰脂肪酶抑制活性，以追踪最有效的提取物。
通过冷浸法制备甲醇提取物及其在极性递增的溶剂（乙醚、氯仿、乙酸乙酯、正丁醇和水）中的馏分。类似地制备乙醇、乙酸乙酯、丙酮和氯仿中的提取物。通过热煎法制备水提取物。采用一种报道的方法测定提取物和馏分在宽浓度范围内的脂肪酶抑制活性。
提取物和馏分表现出浓度依赖性活性。甲醇、乙醇、氯仿、乙酸乙酯、丙酮、水洗后的乙酸乙酯和水煎剂的IC（μg/mL）值分别为293.40、266.47、157.59、182.12、352.34、257.00和190.00。氯仿、乙酸乙酯和水提取物的活性接近药物奥利司他（IC 146 μg/mL）。在甲醇提取物的馏分中，氯仿馏分活性最高（IC 189.6 μg/mL）。馏分的抑制活性顺序如下：氯仿＞乙醚＞正丁醇＞水＞乙酸乙酯。对活性最高的氯仿馏分进行气相色谱/质谱分析，结果显示存在十六烷酸、十六烷酸甲酯、棕榈酸异丙酯、9,12-十八碳二烯酸甲酯和9,12,15-十八碳三烯酸甲酯。
该研究表明，葫芦具有抑制胰脂肪酶活性的潜力，可抑制脂质消化，从而减少脂质进入体内。因此，可能建议定期摄入该果实的水煎剂以控制肥胖。脂肪酸及其酯可能作为脂肪酶抑制剂发挥作用。
