Yu Hong-Hua, Meng Xiao-Wei, Li Jia-Rong, Ouyang Hui, Yin Qian-Qian, Lin Li-Hua, Zhu Wei-Feng, Liu Rong-Hua
School of Pharmacy, Jiangxi University of Traditional Chinese Medicine Nanchang 330004, China.
National Engineering Research Center for Manufacturing Technology of Solid Preparations of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine Nanchang 330004, China.
Zhongguo Zhong Yao Za Zhi. 2022 Jan;47(2):528-536. doi: 10.19540/j.cnki.cjcmm.20210804.201.
UPLC-Q-TOF-MS and serum pharmacochemistry were employed to study the migrating components in rat sera after intragastric administration of the water extracts of Puerariae Lobatae Radix(PLR) and Puerariae Thomsonii Radix(PTR). After the respective intragastric administration of PLR and PTR extracts, blood samples were collected from the orbital vein. The serum samples were treated by protein precipitation method with methanol and acetonitrile at a ratio of 1∶1 and then passed through Agilent ZORBAX RRHD SB-C_(18) column(3 mm×100 mm, 1.8 μm) and Agilent SB-C_(18) pre-column(3 mm×5 mm, 1.8 μm) with 0.1% formic acid aqueous solution(A)-acetonitrile(B) as the mobile phase. The elution was performed at the flow rate of 0.25 mL·min~(-1), the column temperature of 40 ℃, and the injection volume of 2 μL. By comparison of the total ion chromatogram and secondary fragment ion information of PLR and PTR water extracts, PLR-and PTR-containing sera, and blank serum, we found 42 migrating components(including 17 prototype components and 25 metabolites) in the sera of rats treated with PLR and 35 migrating components(including 15 prototype components and 20 metabolites) in the sera of rats treated with PTR. Thirty-three common components were shared by the two treatments, including 13 prototype components and 20 metabolites. The differences of migrating components in the PLR-and PTR-treated rat sera provide a scientific basis for further study of the active components and quality markers of PLR and PTR.
采用超高效液相色谱-四极杆飞行时间质谱联用技术（UPLC-Q-TOF-MS）和血清药物化学方法，研究大鼠灌胃给予葛根（PLR）和甘葛藤（PTR）水提取物后血清中的移行成分。分别灌胃给予PLR和PTR提取物后，从眼眶静脉采集血样。血清样品采用甲醇与乙腈体积比为1∶1的蛋白沉淀法处理，然后通过以0.1%甲酸水溶液（A）-乙腈（B）为流动相的安捷伦ZORBAX RRHD SB-C₁₈柱（3 mm×100 mm，1.8 μm）和安捷伦SB-C₁₈保护柱（3 mm×5 mm，1.8 μm）进行分离。洗脱流速为0.25 mL·min⁻¹，柱温40℃，进样量2 μL。通过比较PLR和PTR水提取物、含PLR和PTR血清以及空白血清的总离子流色谱图和二级碎片离子信息，发现PLR处理组大鼠血清中有42个移行成分（包括17个原型成分和25个代谢产物），PTR处理组大鼠血清中有35个移行成分（包括15个原型成分和20个代谢产物）。两种处理共有33个共同成分，包括13个原型成分和20个代谢产物。PLR和PTR处理的大鼠血清中移行成分的差异为进一步研究PLR和PTR的活性成分及质量标志物提供了科学依据。
