Yue Shi-Yan, Zhou Rong-Rong, Nan Tie-Gui, Huang Lu-Qi, Yuan Yuan
State Key Laboratory Breeding Base of Daodi-herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2022 May;47(10):2689-2697. doi: 10.19540/j.cnki.cjcmm.20220117.202.
For further development and utilization of the germplasm resources of Puerariae Thomsonii Radix and Puerariae Lobatae Radix, this study developed the ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) method, high performance liquid chromatography(HPLC) method, and anthrone colorimetry to detect the content of 23 flavonoids, cellulose, hemicellulose, lignin, soluble sugar, and starch in Puerariae Thomsonii Radix and Puerariae Lobatae Radix. The content differences of various chemical components were analyzed. The methodological test of the established UPLC-MS/MS method for the determination of flavonoids showed that each component had satisfactory linearity within the corresponding linear range(R~2≥0.995), and the average spiked recoveries were 94.48%-105.5%. With this method, 17 flavonoids in Puerariae Lobatae Radix and Puerariae Thomsonii Radix were detected. Based on HPLC and anthrone colorimetry, the determination methods of lignocellulose, soluble sugar, and starch were established. According to the determination results, the content of cellulose in Puerariae Thomsonii Radix was significantly lower than that in Puerariae Lobatae Radix, and the content of starch was significantly higher than that in Puerariae Lobatae Radix. The content of hemicellulose, lignin, and soluble sugar showed no significant difference between the two medicinals, and the content of soluble sugar was in highly significantly negative correlation with that of starch. The established methods are simple, rapid, accurate, and sensitive. The results can lay a basis for the evaluation, and comprehensive development and utilization of the germplasm resources of Puerariae Thomsonii Radix and Puerariae Lobatae Radix.
为进一步开发利用粉葛和野葛的种质资源，本研究建立了超高效液相色谱-串联质谱法（UPLC-MS/MS）、高效液相色谱法（HPLC）和蒽酮比色法，用于检测粉葛和野葛中23种黄酮类化合物、纤维素、半纤维素、木质素、可溶性糖和淀粉的含量。分析了各化学成分的含量差异。所建立的UPLC-MS/MS法测定黄酮类化合物的方法学考察结果表明，各组分在相应线性范围内线性关系良好（R²≥0.995），平均加样回收率为94.48% - 105.5%。采用该方法检测了野葛和粉葛中的17种黄酮类化合物。基于HPLC和蒽酮比色法，建立了木质纤维素、可溶性糖和淀粉的测定方法。根据测定结果，粉葛中纤维素含量显著低于野葛，淀粉含量显著高于野葛。半纤维素、木质素和可溶性糖含量在两种药材中无显著差异，可溶性糖含量与淀粉含量呈极显著负相关。所建立的方法简便、快速、准确、灵敏。研究结果可为粉葛和野葛种质资源的评价及综合开发利用奠定基础。
