College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang Agriculture and Forestry University, Hangzhou, China.
Eur Rev Med Pharmacol Sci. 2022 Feb;26(3):787-798. doi: 10.26355/eurrev_202202_27987.
Silk fibroin (SF) hydrogels are of high interest in tissue engineering. However, angiogenesis is one of the major challenges in tissue regeneration and repair. In this study, we present a simple and effective method to develop a 1,2-Dimyristoyl-sn-glycero-3-phosphorylglycerol sodium salt (DMPG)-SF hydrogel. The SF hydrogels had no immunogenicity and approached natural tissues.
The SF scaffolds were first prepared from Bombyx mori silkworms and DMPG. The SF scaffold was seeded with muscle-derived stem cells derived from sheep embryo and implanted in the tibialis anterior muscle of mature sheep. Gelation time, H&E staining, and histochemistry were conducted and observed. The suitability of the hydrogels for 3D cell culture was assessed by living cell stain CM-Dil.
The results showed that the SF hydrogels resembled the mechanical properties of natural soft tissues better. The results of H&E staining and histochemistry revealed that the degradation rate showed an S-type change, and muscle regeneration and angiogenesis were clearly visible. Adverse effects were not observed in the sheep models.
DMPG-induced SF hydrogels can be successfully used for in situ cell encapsulation. It provides promising opportunities in biomedical applications, such as in tissue engineering and regeneration.
丝素蛋白（SF）水凝胶在组织工程中备受关注。然而，血管生成是组织再生和修复的主要挑战之一。在本研究中，我们提出了一种简单有效的方法来开发 1,2-二肉豆蔻酰基-sn-甘油-3-磷酸甘油钠盐（DMPG）-SF 水凝胶。SF 水凝胶无免疫原性，接近天然组织。
首先从家蚕中制备 SF 支架，然后用 DMPG 处理。将 SF 支架接种来源于绵羊胚胎的肌肉源性干细胞，并植入成熟绵羊的胫骨前肌中。进行凝胶时间、H&E 染色和组织化学检测，并进行观察。通过活细胞染色 CM-Dil 评估水凝胶对 3D 细胞培养的适用性。
结果表明，SF 水凝胶更类似于天然软组织的力学特性。H&E 染色和组织化学结果显示，降解率呈 S 型变化，明显可见肌肉再生和血管生成。绵羊模型中未观察到不良反应。
DMPG 诱导的 SF 水凝胶可成功用于原位细胞包封。它为生物医学应用，如组织工程和再生提供了有前景的机会。
